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Western Blot (WB) (Western BlotPositive WB detected in: Rat brain tissueAll lanes: HIP1R antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120, 71 KDaObserved band size: 120 KDa)

Rabbit anti-Human, Rat HIP1R Polyclonal Antibody | anti-HIP1R antibody

HIP1R Antibody

Gene Names
HIP1R; HIP3; HIP12; ILWEQ
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%, Protein G purified
Synonyms
HIP1R; Polyclonal Antibody; HIP1R Antibody; Huntingtin-interacting protein 1-related protein; HIP1-related protein; Huntingtin-interacting protein 12; HIP-12; HIP12; KIAA0655; anti-HIP1R antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
615
Applicable Applications for anti-HIP1R antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:5000
IHC: 1:500-1:1000
Immunogen
Recombinant Human Huntingtin-interacting protein 1-related protein (304-487AA)
Conjugation
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: Rat brain tissueAll lanes: HIP1R antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120, 71 KDaObserved band size: 120 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat brain tissueAll lanes: HIP1R antibody at 2.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 120, 71 KDaObserved band size: 120 KDa)

Immunohistochemistry (IHC)

(IHC image of MBS7106939 diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7106939 diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of MBS7106939 diluted at 1:800 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of MBS7106939 diluted at 1:800 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Related Product Information for anti-HIP1R antibody
Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
Product Categories/Family for anti-HIP1R antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
70,288 Da
NCBI Official Full Name
huntingtin-interacting protein 1-related protein isoform 2
NCBI Official Synonym Full Names
huntingtin interacting protein 1 related
NCBI Official Symbol
HIP1R
NCBI Official Synonym Symbols
HIP3; HIP12; ILWEQ
NCBI Protein Information
huntingtin-interacting protein 1-related protein
UniProt Protein Name
Huntingtin-interacting protein 1-related protein
UniProt Gene Name
HIP1R
UniProt Synonym Gene Names
HIP12; KIAA0655; HIP1-related protein; HIP-12

Uniprot Description

Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.

Research Articles on HIP1R

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Product Notes

The HIP1R hip1r (Catalog #AAA7106939) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIP1R Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HIP1R can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:5000 IHC: 1:500-1:1000. Researchers should empirically determine the suitability of the HIP1R hip1r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIP1R, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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