Western Blot (WB)
(Western Blot analysis of HeLa cells using HIGD1A Polyclonal Antibody at dilution of 1:500)
Rabbit HIGD1A Polyclonal Antibody | anti-HIGD1A antibody
HIGD1A Antibody
Gene Names
HIGD1A; HIG1; RCF1a
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen affinity purification
Synonyms
HIGD1A; Polyclonal Antibody; HIGD1A Antibody; DKFZp564K247; HIG1; anti-HIGD1A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Sequence Length
93
Applicable Applications for anti-HIGD1A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:3000
IHC: 1:20-1:200
IF: 1:50-1:500
IHC: 1:20-1:200
IF: 1:50-1:500
Immunogen
Fusion protein of HIGD1A
Calculated Molecular Weight
93aa;10kd
Observed Molecular Weight
10kda
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles
Western Blot (WB)
(Western Blot analysis of HeLa cells using HIGD1A Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of HeLa cells using HIGD1A Polyclonal Antibody at dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemistryy of paraffin-embedded human testis, using HIGD1A Polyclonal Antibody at dilution of 1:200)
Immunohistochemistry (IHC)
(Immunohistochemistryy of paraffin-embedded human testis, using HIGD1A Polyclonal Antibody at dilution of 1:200)
Related Product Information for anti-HIGD1A antibody
HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,770 Da
NCBI Official Full Name
HIG1 domain family member 1A, mitochondrial isoform b
NCBI Official Synonym Full Names
HIG1 hypoxia inducible domain family, member 1A
NCBI Official Symbol
HIGD1A
NCBI Official Synonym Symbols
HIG1; RCF1a
NCBI Protein Information
HIG1 domain family member 1A, mitochondrial; HIG1 domain family, member 1A; RCF1 homolog A; hypoxia inducible gene 1; hypoxia-inducible gene 1 protein
UniProt Protein Name
HIG1 domain family member 1A, mitochondrial
Protein Family
UniProt Gene Name
HIGD1A
UniProt Synonym Gene Names
HIG1; RCF1a
UniProt Entry Name
HIG1A_HUMAN
Uniprot Description
HIGD1A: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3p22.1
Cellular Component: protein complex; mitochondrial inner membrane; integral to membrane
Biological Process: cellular response to glucose starvation; negative regulation of apoptosis
Research Articles on HIGD1A
Similar Products
Product Notes
The HIGD1A higd1a (Catalog #AAA2524080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HIGD1A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HIGD1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:3000 IHC: 1:20-1:200 IF: 1:50-1:500. Researchers should empirically determine the suitability of the HIGD1A higd1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIGD1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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