Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HEYL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit anti-Human, Mouse HEYL Polyclonal Antibody | anti-HEYL antibody
HEYL Rabbit pAb
Gene Names
HEYL; HEY3; HRT3; HESR3; bHLHb33
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity purification
Synonyms
HEYL; Polyclonal Antibody; HEYL Rabbit pAb; HEY3; HRT3; HESR3; bHLHb33; anti-HEYL antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
IRYLGVLEGPSSRADPVRIRLLSHLNSYAAEMEPSPTPTGPLAFPAWPWSFFHSCPGLPALSNQLAILGRVPSPVLPGVSSPAYPIPALRTAPLRRATGIILPARRNVLPSRGASSTRRARPLERPATPVPVAPSSRAARSSHIAPLLQSSSPTPPGPTGSAAYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEIGAF
Applicable Applications for anti-HEYL antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 129-328 of human HEYL (NP_055386.1).
Positive Samples
A-549, BxPC-3
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HEYL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HEYL antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Related Product Information for anti-HEYL antibody
Background: This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined.
Product Categories/Family for anti-HEYL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,087 Da
NCBI Official Full Name
hairy/enhancer-of-split related with YRPW motif-like protein
NCBI Official Synonym Full Names
hes-related family bHLH transcription factor with YRPW motif-like
NCBI Official Symbol
HEYL
NCBI Official Synonym Symbols
HEY3; HRT3; HESR3; bHLHb33
NCBI Protein Information
hairy/enhancer-of-split related with YRPW motif-like protein; HEY-like protein; HRT-3; class B basic helix-loop-helix protein 33; hHRT3; hHeyL; hairy-related transcription factor 3; hairy/enhancer-of-split related with YRPW motif 3
UniProt Protein Name
Hairy/enhancer-of-split related with YRPW motif-like protein
Protein Family
UniProt Gene Name
HEYL
UniProt Synonym Gene Names
BHLHB33; HRT3; hHeyL; bHLHb33; HRT-3; hHRT3
UniProt Entry Name
HEYL_HUMAN
Similar Products
Product Notes
The HEYL heyl (Catalog #AAA9142931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HEYL Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HEYL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HEYL heyl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: IRYLGVLEGP SSRADPVRIR LLSHLNSYAA EMEPSPTPTG PLAFPAWPWS FFHSCPGLPA LSNQLAILGR VPSPVLPGVS SPAYPIPALR TAPLRRATGI ILPARRNVLP SRGASSTRRA RPLERPATPV PVAPSSRAAR SSHIAPLLQS SSPTPPGPTG SAAYVAVPTP NSSSPGPAGR PAGAMLYHSW VSEITEIGAF. It is sometimes possible for the material contained within the vial of "HEYL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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