SDS-Page
HEXIM1 recombinant protein
HEXIM1, 1-359aa, Human, His tag, E Coli
Gene Names
HEXIM1; CLP1; EDG1; HIS1; MAQ1
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
HEXIM1; 1-359aa; Human; His tag; E Coli; Hexamethylene bis-acetamide inducible 1; CLP1; EDG1; HIS1; MAQ1; Hexamethylene bis-acetamide inducible 1 Cardiac lineage protein 1; CLP 1; EDG 1; FLJ13562; Hexamethylene bis acetamide inducible 1; Hexamethylene bis acetamide inducible protein; Hexamethylene bis acetamide inducible transcript 1; HEXIM 1; HEXIM1 protein; HIS 1; HMBA inducible; MAQ 1; Menage a quatre 1.; HEXIM1 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD
Sequence Length
359
Applicable Applications for HEXIM1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for HEXIM1 recombinant protein
HEXIM1 is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This protein has been shown to form a protein-RNA complex composed of 7SK small nuclear RNA and positive transcription elongation factor b (P-TEFb), which is composed of cyclin-dependent kinase 9 (CDK9) and cyclin T1, and to inhibit the kinase activity of CDK9, thereby suppressing RNA polymerase II-dependent transcriptional elongation. Recombinant HEXIM1 protein was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for HEXIM1 recombinant protein
References
Shimizu N., et al. (2005) Proc. Natl. Acad. Sci. u.S.A. 102 (24):8555-60; Li Q., et al. (2007) Nucleic Acids Res 35 (8):2503-12.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44.8 kDa (396aa), confirmed by MALDI-TOF.
NCBI Official Full Name
protein HEXIM1
NCBI Official Synonym Full Names
hexamethylene bisacetamide inducible 1
NCBI Official Symbol
HEXIM1
NCBI Official Synonym Symbols
CLP1; EDG1; HIS1; MAQ1
NCBI Protein Information
protein HEXIM1
UniProt Protein Name
Protein HEXIM1
Protein Family
UniProt Gene Name
HEXIM1
UniProt Synonym Gene Names
CLP1; EDG1; HIS1; MAQ1
NCBI Description
Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008]
Uniprot Description
Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor (PubMed:14580347, PubMed:15713661, PubMed:15201869). In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed:12832472, PubMed:14580347, PubMed:15713661, PubMed:15201869). May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity (PubMed:15940264, PubMed:15941832, PubMed:17088550). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).
Research Articles on HEXIM1
Similar Products
Product Notes
The HEXIM1 hexim1 (Catalog #AAA203572) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's HEXIM1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the HEXIM1 hexim1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMAE PFLSEYQHQP QTSNCTGAAA VQEELNPERP PGAEERVPEE DSRWQSRAFP QLGGRPGPEG EGSLESQPPP LQTQACPESS CLREGEKGQN GDDSSAGGDF PPPAEVEPTP EAELLAQPCH DSEASKLGAP AAGGEEEWGQ QQRQLGKKKH RRRPSKKKRH WKPYYKLTWE EKKKFDEKQS LRASRIRAEM FAKGQPVAPY NTTQFLMDDH DQEEPDLKTG LYSKRAAAKS DDTSDDDFME EGGEEDGGSD GMGGDGSEFL QRDFSETYER YHTESLQNMS KQELIKEYLE LEKCLSRMED ENNRLRLESK RLGGDDARVR ELELELDRLR AENLQLLTEN ELHRQQERAP LSKFGD. It is sometimes possible for the material contained within the vial of "HEXIM1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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