Sheep Aquaporin 2, Collecting Duct ELISA Kit | AQP-2 elisa kit

Sheep Aquaporin 2, Collecting Duct ELISA Kit

Cat. Num. AAA737377

• Gene Names: AQP2; AQP-CD; WCH-CD
• Reactivity: Sheep
• Synonyms: Aquaporin 2; Collecting Duct; Sheep Aquaporin 2; Collecting Duct ELISA Kit; AQP-2 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This AQP-2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep AQP-2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-2 elisa kit

Principle of the assay: AQP-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AQP-2 antibody and an AQP-2-HRP conjugate. The assay sample and buffer are incubated together with AQP-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AQP-2 concentration since AQP-2 from samples and AQP-2-HRP conjugate compete for the anti-AQP-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by AQP-2 from the sample, fewer sites are left to bind AQP-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AQP-2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4502179
• NCBI GeneID: 359
• NCBI Accession #: NP_000477.1
• NCBI GenBank Nucleotide #: NM_000486.5
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: aquaporin-2
• NCBI Official Synonym Full Names: aquaporin 2 (collecting duct)
• NCBI Official Symbol: AQP2
• NCBI Official Synonym Symbols: AQP-CD; WCH-CD
• NCBI Protein Information: aquaporin-2; AQP-2; aquaporin-CD; ADH water channel; water-channel aquaporin 2; collecting duct water channel protein; water channel protein for renal collecting duct
• UniProt Protein Name: Aquaporin-2
• UniProt Gene Name: AQP2
• UniProt Synonym Gene Names: AQP-2; AQP-CD
• UniProt Entry Name: AQP2_HUMAN

NCBI Description

This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]

Uniprot Description

AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Channel, misc.; Transporter; Membrane protein, multi-pass; Transporter, aquaporin family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: recycling endosome; protein complex; membrane; rough endoplasmic reticulum; clathrin-coated vesicle; basolateral plasma membrane; lysosome; integral to plasma membrane; early endosome; apical plasma membrane; plasma membrane; trans-Golgi network

Molecular Function: water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; glycerol channel activity; PDZ domain binding

Biological Process: glycerol transport; actin filament depolymerization; cellular water homeostasis; response to glucagon stimulus; apoptosis; response to lithium ion; water transport; response to lipopolysaccharide; cellular response to water deprivation; female pregnancy; excretion; response to salt stress; response to starvation; hyperosmotic response; renal water transport; renal water homeostasis; positive regulation of calcium ion transport; response to calcium ion; transmembrane transport; aging

Disease: Diabetes Insipidus, Nephrogenic, Autosomal