Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protogenin (PRTG) Standard Curve.)
Human Protogenin (PRTG) ELISA Kit | PRTG elisa kit
Human Protogenin (PRTG) ELISA Kit
Gene Names
PRTG; IGDCC5
Reactivity
Human
Synonyms
Protogenin (PRTG); Human Protogenin (PRTG) ELISA Kit; PRTG; FLJ12795; FLJ13221; FLJ25756; IGDCC5; immunoglobulin superfamily; DCC subclass; member 5; protogenin; shen-dan; PRTG elisa kit
Reactivity
Human
Specificity
Human Protogenin (PRTG) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRTG. No significant cross-reactivity or interference between Human PRTG and analogues was observed.
Sequence Length
1150
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protogenin (PRTG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protogenin (PRTG) Standard Curve.)
Related Product Information for PRTG elisa kit
Description: This Human Protogenin (PRTG) ELISA Kit employs a two-site sandwich ELISA to quantitate PRTG in samples. An antibody specific for PRTG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRTG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRTG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRTG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Chicken protogenin contains 1,187 amino acids and has an extracellular domain, followed by 4 immunoglobulin domains and 5 fibronectin type III domains. In situ hybridization showed that expression of chicken protogenin was prominent between embryonic days 1 and 3 and subsequently declined. Transcripts localized to early mesodermal cells, the neuroepithelial cell layer of brain and trunk neural tube, the neural layer of retina, and in the epithelial somite and dermomyotome. By genomic sequence analysis, Toyoda et al. (2005) mapped the human PRTG gene to chromosome 15q21.3. They mapped the mouse Prtg gene to a region of chromosome 9C that shares homology of synteny with human chromosome 15q21.3.
Background: Chicken protogenin contains 1,187 amino acids and has an extracellular domain, followed by 4 immunoglobulin domains and 5 fibronectin type III domains. In situ hybridization showed that expression of chicken protogenin was prominent between embryonic days 1 and 3 and subsequently declined. Transcripts localized to early mesodermal cells, the neuroepithelial cell layer of brain and trunk neural tube, the neural layer of retina, and in the epithelial somite and dermomyotome. By genomic sequence analysis, Toyoda et al. (2005) mapped the human PRTG gene to chromosome 15q21.3. They mapped the mouse Prtg gene to a region of chromosome 9C that shares homology of synteny with human chromosome 15q21.3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protogenin
NCBI Official Synonym Full Names
protogenin
NCBI Official Symbol
PRTG
NCBI Official Synonym Symbols
IGDCC5
NCBI Protein Information
protogenin
UniProt Protein Name
Protogenin
Protein Family
UniProt Gene Name
PRTG
NCBI Description
This gene encodes a member of the immunoglobulin superfamily. The encoded transmembrane protein has been associated with the development of various tissues, especially neurogenesis. It has been suggested that this gene may be associated with attention deficit hyperactivity disorder (ADHD). [provided by RefSeq, Nov 2014]
Uniprot Description
May play a role in anteroposterior axis elongation.
Research Articles on PRTG
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Product Notes
The Human PRTG prtg (Catalog #AAA9716000) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716000 ELISA Kit recognizes Human PRTG. It is sometimes possible for the material contained within the vial of "Protogenin (PRTG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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