Western Blot (WB)
(Western blot analysis of HES5 expression in 293 cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit HES5 Polyclonal Antibody | anti-HES5 antibody
HES5 Antibody
Gene Names
HES5; bHLHb38
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HES5; Polyclonal Antibody; HES5 Antibody; bHLHb38; Class B basic helix-loop-helix protein 38; Hairy and enhancer of split 5; Hairy/Enhancer of spli; Drosophila; homolog of; 5; HES 5; hes family bHLH transcription factor 5; Hes5; HES5 protein; HES5_HUMAN; MGI:104876; Transcription factor HES-5; anti-HES5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HES5 antibody detects endogenous levels of total HES5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
166
Applicable Applications for anti-HES5 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in fetal heart and brain tumors.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Dog (100%), Chicken (82%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of HES5 expression in 293 cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of HES5 expression in 293 cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-HES5 antibody
Function: Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator (By similarity).
Subunit Structure: Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.
Similarity: Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
Subunit Structure: Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.
Similarity: Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 18 kD
Predicted: 19 kDa
Predicted: 19 kDa
NCBI Official Full Name
transcription factor HES-5
NCBI Official Synonym Full Names
hes family bHLH transcription factor 5
NCBI Official Symbol
HES5
NCBI Official Synonym Symbols
bHLHb38
NCBI Protein Information
transcription factor HES-5
UniProt Protein Name
Transcription factor HES-5
Protein Family
UniProt Gene Name
HES5
UniProt Synonym Gene Names
BHLHB38; bHLHb38
NCBI Description
This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer. [provided by RefSeq, Dec 2008]
Uniprot Description
Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator ().
Research Articles on HES5
Similar Products
Product Notes
The HES5 hes5 (Catalog #AAA9603922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HES5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HES5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the HES5 hes5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HES5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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