Rabbit anti-Human RCN1 Polyclonal Antibody | anti-RCN1 antibody

RCN1, CT (RCN1, RCN, Reticulocalbin-1) (Biotin)

Cat. Num. AAA6341061

• Gene Names: RCN1; RCN; RCAL; PIG20; HEL-S-84
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RCN1; Polyclonal Antibody; CT (RCN1; RCN; Reticulocalbin-1) (Biotin); Reticulocalbin-1; anti-RCN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-RCN1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human RCN1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RCN1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RCN1 antibody detected the RCN1 protein (arrow).)

Related Product Information for anti-RCN1 antibody

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.

Product Categories/Family for anti-RCN1 antibody

Antibodies; Proteins

References

Favor, J., et al. Genetics 182(4):1077-1088(2009). Hansen, G.A., et al. Mol. Cell. Biochem. 320 (1-2), 25-33 (2009): Cooper, C.R., et al. J. Cell. Biochem. 104(6):2298-2309(2008). Lim, J., et al. Cell 125(4):801-814(2006). Taylor, T.D., et al. Nature 440(7083):497-500(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4506455
• NCBI GeneID: 5954
• NCBI Accession #: NP_002892.1
• NCBI GenBank Nucleotide #: NM_002901.2
• UniProt Accession #: Q15293; B7Z1M1; D3DR00
• Molecular Weight: 33,026 Da
• NCBI Official Full Name: reticulocalbin-1
• NCBI Official Synonym Full Names: reticulocalbin 1, EF-hand calcium binding domain
• NCBI Official Symbol: RCN1
• NCBI Official Synonym Symbols: RCN; RCAL; PIG20; HEL-S-84
• NCBI Protein Information: reticulocalbin-1; epididymis secretory protein Li 84; proliferation-inducing gene 20
• UniProt Protein Name: Reticulocalbin-1
• Protein Family: Reticulocalbin
• UniProt Gene Name: RCN1
• UniProt Synonym Gene Names: RCN
• UniProt Entry Name: RCN1_HUMAN

NCBI Description

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]

Uniprot Description

RCN1: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: protein binding; calcium ion binding

Biological Process: camera-type eye development; in utero embryonic development