Western Blot (WB)
(Western Blot (WB) analysis of HES-2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate)
Rabbit HES-2 Polyclonal Antibody | anti-HES-2 antibody
HES-2 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
HES-2; Polyclonal Antibody; HES-2 Polyclonal Antibody; Transcription factor HES-2; Class B basic helix-loop-helix protein 40; bHLHb40; Hairy and enhancer of split 2; HES2; BHLHB40; anti-HES-2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
HES-2 polyclonal antibody detects endogenous levels of HES-2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
173
Applicable Applications for anti-HES-2 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to residues in Human HES-2.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of HES-2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate)
Western Blot (WB)
(Western Blot (WB) analysis of HES-2 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse brain tissue lysateLane3:Rat brain tissue lysate)
Related Product Information for anti-HES-2 antibody
The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 18kDa
NCBI Official Full Name
transcription factor HES-2
UniProt Protein Name
Transcription factor HES-2
UniProt Gene Name
HES2
UniProt Synonym Gene Names
BHLHB40; bHLHb40
UniProt Entry Name
HES2_HUMAN
Uniprot Description
HES2: Transcriptional repressor of genes that require a bHLH protein for their transcription. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 1p36.31
Cellular Component: nucleus
Molecular Function: protein dimerization activity; double-stranded DNA binding; transcription factor binding
Biological Process: transcription, DNA-dependent; negative regulation of transcription, DNA-dependent
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Product Notes
The HES-2 hes2 (Catalog #AAA3006533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HES-2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HES-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the HES-2 hes2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HES-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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