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Western Blot (WB)
(Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SH-SY-5Y)
Rabbit HES1 Monoclonal Antibody | anti-HES1 antibody
HES1 Antibody
Gene Names
HES1; HHL; HRY; HES-1; bHLHb39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
HES1; Monoclonal Antibody; HES1 Antibody; bHLHb39 antibody; C-HAIRY1 antibody; c-hairy1A antibody; Class B basic helix-loop-helix protein 39 antibody; FLJ20408 antibody; Hairy and enhancer of split 1 (Drosophila) antibody; Hairy and enhancer of split 1 antibody; Hairy homolog (Drosophila) antibody; Hairy homolog antibody; Hairy like antibody; Hairy; Drosophila; homolog of antibody; Hairy-like protein antibody; Hairy/enhancer of split; homolog of; 1 antibody; HAIRY1 antibody; HES-1 antibody; hes1 antibody; Hes1 hairy and enhancer of split 1 (Drosophila) antibody; HES1_HUMAN antibody; HHL antibody; HL antibody; HRY antibody; MGC129109 antibody; OTTHUMP00000209031 antibody; RHL antibody; Transcription factor HES-1 antibody; anti-HES1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SC06-21
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HES1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SH-SY-5Y)
Western Blot (WB)
(Western blot analysis of Hes1 on different lysates using anti-Hes1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SH-SY-5Y)
Immunocytochemistry (ICC)
(ICC staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Hes1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of sh-sy-5y cells with Hes1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Immunocytochemistry (ICC)
(ICC staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Hes1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Hes1 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-HES1 antibody
The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis. The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis. The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues.
Product Categories/Family for anti-HES1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30kDa
NCBI Official Full Name
transcription factor HES-1
NCBI Official Synonym Full Names
hairy and enhancer of split 1, (Drosophila)
NCBI Official Symbol
HES1
NCBI Official Synonym Symbols
HHL; HRY; HES-1; bHLHb39
NCBI Protein Information
transcription factor HES-1; hairy homolog; hairy-like protein; class B basic helix-loop-helix protein 39
UniProt Protein Name
Transcription factor HES-1
Protein Family
UniProt Gene Name
HES1
UniProt Synonym Gene Names
BHLHB39; HL; HRY; bHLHb39; hHL
UniProt Entry Name
HES1_HUMAN
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Product Notes
The HES1 hes1 (Catalog #AAA9435806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HES1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HES1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the HES1 hes1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HES1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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