Rabbit anti-Human, Mouse Hepcidin 25 Polyclonal Antibody | anti-HAMP antibody

Hepcidin 25 (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Cat. Num. AAA610041

• Gene Names: HAMP; HEPC; PLTR; HFE2B; LEAP1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: Hepcidin 25; Polyclonal Antibody; Hepcidin 25 (Hepc; LEAP; Hepatic Bactericidal Protein; Liver Expressed Antimicrobial Peptide); Anti -Hepcidin 25 (Hepc; anti-HAMP antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Hepcidin 25. The mouse sequences from mouse/rat and human differ by just 1aa in 25aa mature HEPC. The sequence is truncated by 5aa in the 20aa major secreted HEPC. No significant crossreactivity with 20aa HEPC peptide. May crossreacts with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-HAMP antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000 (ECL).; ELISA: 1:100,000 using 50-100ng control peptide/well.
• Immunogen: Synthetic peptides consisting of 7aa sequence from the N-terminus of mature human and mouse 25aa HEPC (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-HAMP antibody

Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues. In humans, 20aa and 25aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).

Product Categories/Family for anti-HAMP antibody

Antibodies; Abs to Peptides

NCBI and Uniprot Product Information

• NCBI GI #: 10863973
• NCBI GeneID: 57817
• NCBI Accession #: NP_066998.1
• NCBI GenBank Nucleotide #: NM_021175.2
• UniProt Accession #: P81172; Q1HE14; Q9BY68
• Molecular Weight: 9,408 Da
• NCBI Official Full Name: hepcidin preproprotein
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: HAMP
• NCBI Official Synonym Symbols: HEPC; PLTR; HFE2B; LEAP1
• NCBI Protein Information: hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
• UniProt Protein Name: Hepcidin
• Protein Family: Hepcidin
• UniProt Gene Name: HAMP
• UniProt Synonym Gene Names: HEPC; LEAP1; LEAP-1; PLTR; Hepc25; Hepc20
• UniProt Entry Name: HEPC_HUMAN

NCBI Description

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]

Uniprot Description

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: killing of cells of another organism; cellular iron ion homeostasis; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b