Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human Hepatocyte Nuclear Factor 4 Alpha (HNF4a) Polyclonal Antibody | anti-HNF4a antibody
Polyclonal Antibody to Hepatocyte Nuclear Factor 4 Alpha (HNF4a)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QNERDRISTR RSSYEDSSLP SINALLQAEV LSRQITSPVS GINGDIRAKK IASIADVCES MKEQLLVLVE WAKYIPAFCE LPLDDQVALL RAHAGEHLLL GATKRSMVFK DVLLLGNDYI VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYAYLK AIIFFDPDAK GLSDPGKIKR LRSQVQVSLE DYINDRQYDS
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
Uniprot Description
HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.
Protein type: DNA-binding; Nuclear receptor; Transcription factor
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: cytoplasm; nucleoplasm; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; fatty acid binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; receptor binding; steroid hormone receptor activity; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding; transcription regulatory region DNA binding; zinc ion binding
Biological Process: blood coagulation; glucose homeostasis; intracellular receptor signaling pathway; lipid homeostasis; lipid metabolic process; negative regulation of cell growth; negative regulation of cell proliferation; ornithine metabolic process; phospholipid homeostasis; positive regulation of cholesterol homeostasis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of gastrulation; regulation of insulin secretion; regulation of lipid metabolic process; regulation of transcription from RNA polymerase II promoter; response to glucose stimulus; sex differentiation; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter; triglyceride homeostasis; xenobiotic metabolic process
Disease: Diabetes Mellitus, Noninsulin-dependent; Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1
Research Articles on HNF4a
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Product Notes
The HNF4a hnf4a (Catalog #AAA2015303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Hepatocyte Nuclear Factor 4 Alpha (HNF4a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hepatocyte Nuclear Factor 4 Alpha (HNF4a) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HNF4a hnf4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QNERDRISTR RSSYEDSSLP SINALLQAEV LSRQITSPVS GINGDIRAKK IASIADVCES MKEQLLVLVE WAKYIPAFCE LPLDDQVALL RAHAGEHLLL GATKRSMVFK DVLLLGNDYI VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYAYLK AIIFFDPDAK GLSDPGKIKR LRSQVQVSLE DYINDRQYDS. It is sometimes possible for the material contained within the vial of "Hepatocyte Nuclear Factor 4 Alpha (HNF4a), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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