Rabbit Hemoglobin subunit alpha Polyclonal Antibody | anti-Hba-a1 antibody

Rabbit anti-mouse Hemoglobin subunit alpha polyclonal Antibody

Cat. Num. AAA1489797

• Gene Names: Hba-a1; Hba; Hba1; Hbat1
• Reactivity: Mouse, Human. Other species are not tested. Please decide the specificity by homology.
• Applications: ELISA, Western Blot
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Hemoglobin subunit alpha; Polyclonal Antibody; Rabbit anti-mouse Hemoglobin subunit alpha polyclonal Antibody; Alpha-globin Hemoglobin alpha chain Hba Hba-a1; anti-Hba-a1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human. Other species are not tested. Please decide the specificity by homology.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 142

• Applicable Applications for anti-Hba-a1 antibody: ELISA (EIA), Western Blot (WB)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant mouse Hemoglobin subunit alpha protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-31332
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Hemoglobin subunit alpha antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45kDaObserved band size: 45kDa)

NCBI and Uniprot Product Information

• NCBI GI #: 122441
• NCBI GeneID: 15122
• UniProt Accession #: P01942
• Molecular Weight: 15,085 Da
• NCBI Official Full Name: Hemoglobin subunit alpha
• NCBI Official Synonym Full Names: hemoglobin alpha, adult chain 1
• NCBI Official Symbol: Hba-a1
• NCBI Official Synonym Symbols: Hba; Hba1; Hbat1
• NCBI Protein Information: hemoglobin subunit alpha
• UniProt Protein Name: Hemoglobin subunit alpha
• Protein Family: Hemoglobin
• UniProt Gene Name: Hba
• UniProt Synonym Gene Names: Hba-a1
• UniProt Entry Name: HBA_MOUSE

Uniprot Description

HBA1: Involved in oxygen transport from the lung to the various peripheral tissues. Defects in HBA1 may be a cause of Heinz body anemias (HEIBAN). This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency. Defects in HBA1 are the cause of alpha-thalassemia (A- THAL). The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non- deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers. Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non- immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. Defects in HBA1 are the cause of hemoglobin H disease (HBH). HBH is a form of alpha-thalassemia due to the loss of three alpha genes. This results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia. Untreated, most patients die in childhood or early adolescence. Belongs to the globin family.

Protein type: Carrier

Cellular Component: hemoglobin complex; membrane

Molecular Function: haptoglobin binding; heme binding; organic acid binding; oxygen binding; peroxidase activity

Biological Process: erythrocyte development; in utero embryonic development