Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Heme Oxygenase 2 Polyclonal Antibody | anti-HO2 antibody
Polyclonal Antibody to Heme Oxygenase 2, Decycling (HO2)
Gene Names
Hmox2; HO2; HO-2
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Heme Oxygenase 2; Polyclonal Antibody; Polyclonal Antibody to Heme Oxygenase 2; Decycling (HO2); anti-HO2 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against HO2. It has beenselected for its ability to recognize HO2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KEAHDRAENT QFVKDFLKGN IKKELFKLAT TALYFTYSAL EEEMDRNKDH PAFAPLYFPT ELHRKAALIK DMKYFFGENW EEQVKCSEAA QKYVDRIHYV GQNEPELLVA HAYTRYMGDL SGGQVLKKVA QRALKLPSTG EGTQFYLFEH VDNAQQFKQF YRARMNALDL NLKTKERIVE EANKAFEYNM QIFSELDQAG SMLARETLED GLPVHDGKGD IRKCPFYAAQ PDKGTLGGSN CPFQTTVAVL RK
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KEAHDRAENT QFVKDFLKGN IKKELFKLAT TALYFTYSAL EEEMDRNKDH PAFAPLYFPT ELHRKAALIK DMKYFFGENW EEQVKCSEAA QKYVDRIHYV GQNEPELLVA HAYTRYMGDL SGGQVLKKVA QRALKLPSTG EGTQFYLFEH VDNAQQFKQF YRARMNALDL NLKTKERIVE EANKAFEYNM QIFSELDQAG SMLARETLED GLPVHDGKGD IRKCPFYAAQ PDKGTLGGSN CPFQTTVAVL RK
Sequence Length
312
Applicable Applications for anti-HO2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
HO2 (Lys41~Lys292)
Organism Species
Mus musculus (Mouse)
Cross Reactivity
Mouse
Conjugate
No Conjugate
Immunogen
Recombinant HO2 (Lys41~Lys292) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2044646
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Lung tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Lung tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Liver Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,739 Da
NCBI Official Full Name
heme oxygenase 2 isoform 1
NCBI Official Synonym Full Names
heme oxygenase 2
NCBI Official Symbol
Hmox2
NCBI Official Synonym Symbols
HO2; HO-2
NCBI Protein Information
heme oxygenase 2
UniProt Protein Name
Heme oxygenase 2
Protein Family
UniProt Gene Name
Hmox2
UniProt Synonym Gene Names
HO-2
Uniprot Description
HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.
Protein type: Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3; Endoplasmic reticulum; Oxidoreductase
Chromosomal Location of Human Ortholog: 16 A1|16 2.46 cM
Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; membrane; plasma membrane
Molecular Function: heme binding; heme oxygenase (decyclizing) activity; metal ion binding; oxidoreductase activity
Biological Process: heme catabolic process; heme oxidation; iron ion homeostasis; response to hypoxia; response to oxidative stress
Research Articles on HO2
Similar Products
Product Notes
The HO2 hmox2 (Catalog #AAA2026011) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Heme Oxygenase 2, Decycling (HO2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Heme Oxygenase 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the HO2 hmox2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KEAHDRAENT QFVKDFLKGN IKKELFKLAT TALYFTYSAL EEEMDRNKDH PAFAPLYFPT ELHRKAALIK DMKYFFGENW EEQVKCSEAA QKYVDRIHYV GQNEPELLVA HAYTRYMGDL SGGQVLKKVA QRALKLPSTG EGTQFYLFEH VDNAQQFKQF YRARMNALDL NLKTKERIVE EANKAFEYNM QIFSELDQAG SMLARETLED GLPVHDGKGD IRKCPFYAAQ PDKGTLGGSN CPFQTTVAVL RK. It is sometimes possible for the material contained within the vial of "Heme Oxygenase 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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