Rabbit anti-Human, Mouse FGF9 Polyclonal Antibody | anti-FGF9 antibody

FGF9, NT (FGF9, Fibroblast growth factor 9, Glia-activating factor, Heparin-binding growth factor 9) (APC)

Cat. Num. AAA6299056

• Gene Names: FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FGF9; Polyclonal Antibody; NT (FGF9; Fibroblast growth factor 9; Glia-activating factor; Heparin-binding growth factor 9) (APC); Heparin-binding growth factor 9; anti-FGF9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human FGF9. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-FGF9 antibody: Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 31-58 in the N-terminal region of human FGF9, UniProt Accession #P31371. Species sequence homology: porcine and rat
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MBS6003592 in mouse kidney tissue lysates (35ug/lane). This demonstrates the FGF9 antibody detected the FGF9 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of FGF9 (arrow) usingMBS6003592. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FGF9 gene.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MBS6003592 using MCF-7 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-FGF9 antibody

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.

Product Categories/Family for anti-FGF9 antibody

Antibodies; Growth Factors; Cytokines

References

1. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 2. Yokoyama, K., et al. Nephron Clin Pract 115 (4), C237-C243 (2010). 3. Kalinina, J., et al. Mol. Cell. Biol. 29(17):4663-4678(2009). 4. Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009). 5. Wu, X.L., et al. Am. J. Hum. Genet. 85(1):53-63(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503707
• NCBI GeneID: 2254
• NCBI Accession #: NP_002001.1
• NCBI GenBank Nucleotide #: NM_002010.2
• UniProt Accession #: P31371; Q3SY32; A8K427
• Molecular Weight: 23,441 Da
• NCBI Official Full Name: fibroblast growth factor 9
• NCBI Official Synonym Full Names: fibroblast growth factor 9
• NCBI Official Symbol: FGF9
• NCBI Official Synonym Symbols: GAF; FGF-9; SYNS3; HBFG-9; HBGF-9
• NCBI Protein Information: fibroblast growth factor 9; heparin-binding growth factor 9; fibroblast growth factor 9 (glia-activating factor)
• UniProt Protein Name: Fibroblast growth factor 9
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF9
• UniProt Synonym Gene Names: FGF-9; GAF; HBGF-9
• UniProt Entry Name: FGF9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF9: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3). Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and progressive conductive deafness. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 13q11-q12

Cellular Component: extracellular space; cytoplasm; extracellular region; basement membrane

Molecular Function: heparin binding; growth factor activity; fibroblast growth factor receptor binding

Biological Process: negative regulation of Wnt receptor signaling pathway; nerve growth factor receptor signaling pathway; embryonic skeletal development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of activin receptor signaling pathway; substantia nigra development; positive regulation of MAPKKK cascade; cell-cell signaling; protein import into nucleus; male sex determination; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; chondrocyte differentiation; positive regulation of smoothened signaling pathway; angiogenesis; embryonic gut development; regulation of timing of cell differentiation; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; male gonad development; osteoblast differentiation; positive regulation of cell division; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation

Disease: Multiple Synostoses Syndrome 3