Heme Oxygenase 2 Recombinant Protein | HO2 recombinant protein

Recombinant Heme Oxygenase 2, Decycling (HO2)

Cat. Num. AAA2031162

• Gene Names: Hmox2; HO2; HO-2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Heme Oxygenase 2; Recombinant Heme Oxygenase 2; Decycling (HO2); HO2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 7.3 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- KEAHDRAENT QFVKDFLKGN IKKELFKLAT TALYFTYSAL EEEMDRNKDH PAFAPLYFPT ELHRKAALIK DMKYFFGENW EEQVKCSEAA QKYVDRIHYV GQNEPELLVA HAYTRYMGDL SGGQVLKKVA QRALKLPSTG EGTQFYLFEH VDNAQQFKQF YRARMNALDL NLKTKERIVE EANKAFEYNM QIFSELDQAGSMLARETLED GLPVHDGKGD IRKCPFYAAQ PDKGTLGGSN CPFQTTVAVL RK
• Sequence Length: 315

• Applicable Applications for HO2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.7kDa
• Accurate Molecular Mass (KD): 33kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Lys41~Lys292
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 225007593
• NCBI GeneID: 15369
• NCBI Accession #: NP_034573.2
• NCBI GenBank Nucleotide #: NM_010443.2
• Molecular Weight: 35,739 Da
• NCBI Official Full Name: heme oxygenase 2
• NCBI Official Synonym Full Names: heme oxygenase 2
• NCBI Official Symbol: Hmox2
• NCBI Official Synonym Symbols: HO2; HO-2
• NCBI Protein Information: heme oxygenase 2
• UniProt Protein Name: Heme oxygenase 2
• Protein Family: Heme oxygenase
• UniProt Gene Name: Hmox2
• UniProt Synonym Gene Names: HO-2
• UniProt Entry Name: HMOX2_MOUSE

Uniprot Description

HMOX2: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Belongs to the heme oxygenase family.

Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.14.99.3

Cellular Component: membrane; intracellular membrane-bound organelle; endoplasmic reticulum; plasma membrane

Molecular Function: metal ion binding; oxidoreductase activity; heme oxygenase (decyclizing) activity

Biological Process: heme oxidation; response to hypoxia; response to oxidative stress