Human GIF ELISA Kit | GIF elisa kit

Human GIF PicoKine ELISA Kit

Cat. Num. AAA1752607

• Gene Names: CBLIF; IF; GIF; INF; IFMH; TCN3
• Reactivity: Human
• Synonyms: GIF; Human GIF PicoKine ELISA Kit; Human GIF; Cobalamin binding intrinsic factor; Gastric intrinsic factor; Intrinsic factor; IF; INF; CBLIF; GIF elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human CBLIF
• Sequence Length: 417

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GIF elisa kit

Principle of the Assay: The Picokine™ Human CBLIF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CBLIF with a 96-well strip plate that is pre-coated with antibody specific for CBLIF. The detection antibody is a biotinylated antibody specific for CBLIF. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human CBLIF with immunogen: Expression system for standard: NSO, Immunogen sequence: S19-Y417. The kit is analytically validated with ready to use reagents. To measure Human CBLIF, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human CBLIF in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CBLIF in the sample.!!Background/Introduction: Intrinsic factor (IF), also known as gastric intrinsic factor (GIF), is a glycoprotein produced by the parietal cells of the stomach. It is necessary for the absorption of vitamin B12 later on in the ileum of the small intestine. In humans, the gastric intrinsic factor protein is encoded by the GIF gene. It is mapped to 11q12.1. Haptocorrin (also known as HC, R protein, and transcobalamin I, TCN1) is another glycoprotein secreted by the salivary glands which binds to vitamin B12. Vitamin B12 is acid sensitive and in binding to transcobalamin I it can safely pass through the acidic stomach to the duodenum. In the less acidic environment of the small intestine, pancreatic enzymes digest the glycoprotein carrier and vitamin B12 can then bind to intrinsic factor

NCBI and Uniprot Product Information

• NCBI GI #: 62906845
• NCBI GeneID: 2694
• UniProt Accession #: P27352
• NCBI Official Full Name: Cobalamin binding intrinsic factor
• NCBI Official Synonym Full Names: cobalamin binding intrinsic factor
• NCBI Official Symbol: CBLIF
• NCBI Official Synonym Symbols: IF; GIF; INF; IFMH; TCN3
• NCBI Protein Information: cobalamin binding intrinsic factor
• UniProt Protein Name: Gastric intrinsic factor
• Protein Family: Gastric intrinsic factor
• UniProt Gene Name: GIF
• UniProt Synonym Gene Names: IFMH; IF; INF
• UniProt Entry Name: IF_HUMAN

NCBI Description

This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008]

Uniprot Description

GIF: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. Defects in GIF are the cause of hereditary intrinsic factor deficiency (IFD); also known as congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; lysosomal lumen; microvillus; apical plasma membrane; extracellular region; endosome

Molecular Function: cobalamin binding

Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process

Disease: Intrinsic Factor Deficiency