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Western Blot (WB) (Western Blot analysis in COS7 cells (15ug/lane) using.)

Mouse Hemagglutinin Tag Monoclonal Antibody

Hemagglutinin Tag (HA Tag) (AP)

Applications
ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
Hemagglutinin Tag; Monoclonal Antibody; Hemagglutinin Tag (HA Tag) (AP); anti-Hemagglutinin Tag antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
12CA5
Specificity
Recognizes Hemagglutinin Tag.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Hemagglutinin Tag antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide encoding Hemagglutinin Tag (YPYDVPDYA).
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in COS7 cells (15ug/lane) using.)

Western Blot (WB) (Western Blot analysis in COS7 cells (15ug/lane) using.)

Western Blot (WB)

(Western Blot analysis of in 12-tag recombinant protein using.)

Western Blot (WB) (Western Blot analysis of in 12-tag recombinant protein using.)
Related Product Information for anti-Hemagglutinin Tag antibody
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Our anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.
Product Categories/Family for anti-Hemagglutinin Tag antibody
References
1. Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. 2. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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Product Notes

The Hemagglutinin Tag (Catalog #AAA6120837) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hemagglutinin Tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Hemagglutinin Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemagglutinin Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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