Rabbit HELLS Polyclonal Antibody | anti-HELLS antibody

HELLS (Lymphoid-specific Helicase, Proliferation-associated SNF2-like Protein, SWI/SNF2-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 6, PASG, SMARCA6, Nbla10143)

Cat. Num. AAA643271

• Gene Names: HELLS; LSH; PASG; SMARCA6; Nbla10143
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HELLS; Polyclonal Antibody; HELLS (Lymphoid-specific Helicase; Proliferation-associated SNF2-like Protein; SWI/SNF2-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A Member 6; PASG; SMARCA6; Nbla10143); Anti -HELLS (Lymphoid-specific Helicase; anti-HELLS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human HELLS. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-HELLS antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot and Immunocytochemistry.; Dilution: Immunocytochemistry: 2ug/ml; Western Blot: 0.1ug/ml
• Immunogen: Synthetic linear peptide corresponding to he coiled-coil region of human Lymphoid-specific helicase (KLH).
• Positive Control: HeLa nuclear extract
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-HELLS antibody

A member of the SNF2 family of chromatin remodeling proteins, Lymphoid-specific helicase (HELLS, also known as Lsh) assumes various roles in normal development, survival, de novo or maintenance DNA methylation, gene silencing, and heterochromatin formation and organization. It is shown to serve as cancer progression marker in conjunction with CEP55 and FOXM1 for the detection of head and neck squamous cell carcinoma. It is also reported that expression of Lsh is crucial for meiotic progression in mouse spermatocytes. Lsh is shown to directly associate with the 5' DMR of the Cdkn1c promoter, and thereby regulate its gene expression. Additionally, Lsh is reported as a regulator of repressive chromatin at retrotransposons, and is demonstrated to mediate the silencing of stem cell-specific genes such as Oct4.

Product Categories/Family for anti-HELLS antibody

Antibodies; Abs to Chromatin Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 32425420
• NCBI GeneID: 3070
• UniProt Accession #: Q9NRZ9; Q3LID1; Q6I7N7; Q76H76; Q76H77; Q76H78; Q76H79; Q76H80; Q76H81; Q7Z397; Q7Z5X2; B2RB41
• Molecular Weight: 97,074 Da
• NCBI Official Full Name: HELLS protein, partial
• NCBI Official Synonym Full Names: helicase, lymphoid-specific
• NCBI Official Symbol: HELLS
• NCBI Official Synonym Symbols: LSH; PASG; SMARCA6; Nbla10143
• NCBI Protein Information: lymphoid-specific helicase; proliferation-associated SNF2-like protein; SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6; SWI/SNF2-related, matrix-associated, actin-dependent regulator of chromatin, subfamily A, member 6
• UniProt Protein Name: Lymphoid-specific helicase
• Protein Family: Lymphoid-specific helicase
• UniProt Gene Name: HELLS
• UniProt Synonym Gene Names: PASG; SMARCA6
• UniProt Entry Name: HELLS_HUMAN

NCBI Description

This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HELLS: Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis. By concanavalin-A in peripheral blood leukocytes. Highly expressed in proliferative tissues such as adult thymus and testis, and expressed at lower levels in uterus, small intestine, colon, and peripheral blood mononuclear cells. Also expressed in neoplastic cell lines including those derived from myeloid and lymphoid leukemias. Belongs to the SNF2/RAD54 helicase family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.6.1.-; EC 3.6.4.-; Helicase

Chromosomal Location of Human Ortholog: 10q24.2

Cellular Component: centric heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; helicase activity; ATP binding

Biological Process: mitosis; lymphocyte proliferation; transcription, DNA-dependent; methylation-dependent chromatin silencing; maintenance of DNA methylation; cell division; multicellular organismal development; centric heterochromatin formation