Western Blot (WB)
(Anti- HEF1 Picoband antibody, MBS178099, Western blottingAll lanes: Anti HEF1 (MBS178099) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: CEM Whole Cell Lysate at 40ugLane 3: RAJI Whole Cell Lysate at 40ugPredicted bind size: 93KDObserved bind size: 93KD)
anti-Human HEF1 Polyclonal Antibody | anti-HEF1 antibody
Anti-HEF1 Antibody
Western Blot (WB)
(Anti- HEF1 Picoband antibody, MBS178099, Western blottingAll lanes: Anti HEF1 (MBS178099) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: CEM Whole Cell Lysate at 40ugLane 3: RAJI Whole Cell Lysate at 40ugPredicted bind size: 93KDObserved bind size: 93KD)
Western Blot (WB)
(Anti- HEF1 Picoband antibody, MBS178099, Western blottingAll lanes: Anti HEF1 (MBS178099) at 0.5ug/mlLane 1: JURKAT Whole Cell Lysate at 40ugLane 2: CEM Whole Cell Lysate at 40ugLane 3: RAJI Whole Cell Lysate at 40ugPredicted bind size: 93KDObserved bind size: 93KD)
Background: Enhancer of filamentation 1 (EF1), also known as NEDD-9, is a protein that in humans is encoded by the NEDD9 gene. The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. It is mapped to 6p24.2. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. NEDD9 is also a highly relevant cancer gene that governs metastatic potential in human melanoma. What's more, it has been speculated that NEDD9 may be an important linking element between extracellular signaling and regulation of the cytoskeleton.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Uniprot Description
Cas-L: a widely expressed docking protein which plays a central coordinating role for tyrosine-kinase-based signaling in cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. Phosphorylated following integrin beta-1, antigen receptor, or C1a calcitonin receptor signaling. Transformation of fibroblasts with v-ABL results in an increase in its tyrosine phosphorylation. Phosphorylated by focal adhesion kinase. Highly expressed in kidney, lung, and placenta. Also detected in T-cells, B-cells and diverse cell lines.
Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 6p24.2
Cellular Component: cell cortex; cytoplasm; focal adhesion; Golgi apparatus; lamellipodium; nucleus; spindle; spindle pole
Molecular Function: protein binding
Biological Process: actin filament bundle formation; cell adhesion; cell division; cytoskeleton organization and biogenesis; integrin-mediated signaling pathway; mitosis; regulation of growth; signal transduction
Research Articles on HEF1
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Product Notes
The HEF1 nedd9 (Catalog #AAA178099) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-HEF1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HEF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the HEF1 nedd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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