Rabbit Mineralocorticoid Receptor Polyclonal Antibody | anti-NR3C2 antibody

Mineralocorticoid Receptor Antibody

Cat. Num. AAA9612061

• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish(83%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(88%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Mineralocorticoid Receptor; Polyclonal Antibody; Mineralocorticoid Receptor Antibody; Aldosterone receptor; MCR; MCR_HUMAN; MGC133092; Mineralocorticoid receptor; MLR; MR; NR3 C2; NR3C2; NR3C2 protein; Nuclear receptor subfamily 3 group C member 2; anti-NR3C2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Zebrafish(83%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(88%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mineralocorticoid Receptor Antibody detects endogenous levels of total Mineralocorticoid Receptor.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 984

• Applicable Applications for anti-NR3C2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Mineralocorticoid Receptor, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain, using Mineralocorticoid Receptor Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-NR3C2 antibody

Function: Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.

Post Translational Modifications: Phosphorylated.

Subcellular Location: Cytoplasm. Nucleus. Endoplasmic reticulum membrane>Peripheral membrane protein. Note: Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.

Tissue Specificity: Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.

Subunit Structure: Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP4, in the absence of ligand. After ligand binding, it translocates to the nucleus and binds to DNA as a homodimer and as a heterodimer with NR3C1. May interact with HSD11B2 in the absence of ligand. Binds the coactivators NCOA1, NCOA2, TIF1 and NRIP1.

Similarity: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.Belongs to the nuclear hormone receptor family. NR3 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 126885
• UniProt Accession #: P08235
• Molecular Weight: Observed Molecular Weight: (Observed)107kD.; Predicted Molecular Weight: (Calculated)107kDa.
• NCBI Official Full Name: Mineralocorticoid receptor
• UniProt Protein Name: Mineralocorticoid receptor
• Protein Family: Mineralocorticoid receptor
• UniProt Gene Name: NR3C2
• UniProt Synonym Gene Names: MCR; MLR; MR
• UniProt Entry Name: MCR_HUMAN

Uniprot Description

MCR: Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels. Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1). PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported. Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP). Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. Belongs to the nuclear hormone receptor family. NR3 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 4q31.1

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; receptor complex

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription factor activity; steroid binding

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression; steroid hormone mediated signaling; signal transduction

Disease: Hypertension, Early-onset, Autosomal Dominant, With Severe Exacerbation In Pregnancy; Pseudohypoaldosteronism, Type I, Autosomal Dominant