Western Blot (WB)
(Western blotAll lanes: Hsp90ab1antibody at 2ug/mlLane 1: Mouse lung tissueLane 2: Rat lung tissueLane 3: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 83 kDaObserved band size: 83,120,32 kDa)
Rabbit anti-Human Heat shock protein HSP 90-beta Polyclonal Antibody | anti-HSP90B antibody
Rabbit anti-human Heat shock protein HSP 90-beta polyclonal Antibody
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western blotAll lanes: Hsp90ab1antibody at 2ug/mlLane 1: Mouse lung tissueLane 2: Rat lung tissueLane 3: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 83 kDaObserved band size: 83,120,32 kDa)
Western Blot (WB)
(Western blotAll lanes: Hsp90ab1antibody at 2ug/mlLane 1: Mouse lung tissueLane 2: Rat lung tissueLane 3: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 83 kDaObserved band size: 83,120,32 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: hepG2 cellAll lanes: CHD4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 83 kDaObserved band size: 83 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: hepG2 cellAll lanes: CHD4 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 83 kDaObserved band size: 83 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: HSP90AB1 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 84 KDaObserved band size: 84 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysateAll lanes: HSP90AB1 antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 84 KDaObserved band size: 84 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS1499004 diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS1499004 diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF-7 cells with MBS1499004 at 1:66,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of MCF-7 cells with MBS1499004 at 1:66,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012]
Uniprot Description
HSP90B: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4. Belongs to the heat shock protein 90 family.
Protein type: Heat shock protein; Chaperone
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: apical plasma membrane; basolateral plasma membrane; brush border membrane; cell surface; cytoplasm; cytosol; inclusion body; melanosome; membrane; mitochondrion; nucleoplasm; signalosome
Molecular Function: ATP binding; dATP binding; double-stranded RNA binding; GTP binding; histone deacetylase binding; kinase binding; nitric-oxide synthase regulator activity; protein binding; protein kinase binding; sulfonylurea receptor binding; TPR domain binding; unfolded protein binding
Biological Process: axon guidance; innate immune response; negative regulation of neuron apoptosis; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; placenta development; positive regulation of cell size; positive regulation of nitric oxide biosynthetic process; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; protein folding; regulation of protein ubiquitination; response to salt stress; response to unfolded protein; virion attachment to host cell surface receptor
Research Articles on HSP90B
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Product Notes
The HSP90B hsp90ab1 (Catalog #AAA1499004) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Heat shock protein HSP 90-beta polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Heat shock protein HSP 90-beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the HSP90B hsp90ab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat shock protein HSP 90-beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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