Goat Heat Shock Cognate Protein 71 Polyclonal Antibody | anti-HSPA8 antibody

Heat Shock Cognate Protein 71 (HSC71, HSPA8)

Cat. Num. AAA615457

• Gene Names: HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10; MGC29929; MGC131511
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Heat Shock Cognate Protein 71; Polyclonal Antibody; Heat Shock Cognate Protein 71 (HSC71; HSPA8); Anti -Heat Shock Cognate Protein 71 (HSC71; anti-HSPA8 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous human, mouse, and rat HSPA8/HSC71, Isoform 1 in Western Blots. Does not crossreact with recombinant human HSPA1A /HSP70, HSPA2, HSPA6, GRP75 or GRP78 in Western Blots.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol.

• Applicable Applications for anti-HSPA8 antibody: Western Blot (WB)
• Application Notes: Western Blot: An antibody concentration of 1.0ug/ml is recommended., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: E. coli-derived recombinant human HSPA8 (aa534-615)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HSPA8 antibody

The 70kD heat shock proteins (HSP70s) are a highly conserved family of stress response proteins. The HSP70 family of proteins contains both heat/stress inducible and constitutively expressed members known as heat shock cognate proteins. Heat Shock 70 kD Protein 8 (HSPA8), also known as Heat Shock Cognate Protein 71 (HSC71), HSC70, HSP73, HSPA10 and LPS-associated protein 1 (LAP1), is a 646 amino acid (aa) heat shock cognate protein. Many HSPs function as molecular chaperones, facilitating the folding of other cellular proteins. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSPA8 is a ubiquitously expressed protein and rapidly translocates from the cytoplasm to the nucleus in response to heat shock. Two alternatively spliced variants have been identified. This antibody detects isoform 1 but not isoform 2 (HSC54). Human HSPA8 shares greater than 99 % identity with mouse and rat HSPA8.

Product Categories/Family for anti-HSPA8 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24234686
• NCBI GeneID: 3312
• NCBI Accession #: NP_694881.1
• NCBI GenBank Nucleotide #: NM_153201.2
• UniProt Accession #: Q53HF2; P11142; Q53GZ6; Q96BE0; Q96H53; Q96IS6; Q9NWW3; Q9NZ87
• Molecular Weight: 53,500 Da
• NCBI Official Full Name: heat shock cognate 71 kDa protein isoform 2
• NCBI Official Synonym Full Names: heat shock 70kDa protein 8
• NCBI Official Symbol: HSPA8
• NCBI Official Synonym Symbols: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10; MGC29929; MGC131511
• NCBI Protein Information: heat shock cognate 71 kDa protein; OTTHUMP00000231743; OTTHUMP00000231744; OTTHUMP00000231745; OTTHUMP00000231746; OTTHUMP00000231747; OTTHUMP00000231749; LPS-associated protein 1; heat shock 70kd protein 10; heat shock cognate protein 54; constitutive heat shock protein 70; lipopolysaccharide-associated protein 1; N-myristoyltransferase inhibitor protein 71
• UniProt Protein Name: Heat shock 70kDa protein 8 isoform 2 variant
• UniProt Entry Name: Q53HF2_HUMAN

NCBI Description

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date. [provided by RefSeq]

Uniprot Description

HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Heat shock protein; RNA-binding; Chaperone; Nucleolus

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: nucleoplasm; spliceosome; extracellular space; focal adhesion; membrane; melanosome; plasma membrane; nucleolus; intracellular; nucleus; cytosol; ribonucleoprotein complex; ubiquitin ligase complex

Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; heat shock protein binding; ATPase activity; unfolded protein binding; ATPase activity, coupled; ATP binding

Biological Process: axon guidance; protein folding; chaperone cofactor-dependent protein folding; viral reproduction; transcription, DNA-dependent; regulation of cell cycle; neurotransmitter secretion; RNA splicing; response to unfolded protein; positive regulation of nuclear mRNA splicing, via spliceosome; nuclear mRNA splicing, via spliceosome; synaptic transmission; gene expression; protein refolding; post-Golgi vesicle-mediated transport; negative regulation of transcription, DNA-dependent