Western Blot (WB)
(Sample Data: Representative western blot with Anti-HDAC5 (1:1000) using 25 ng of human recombinant proteins HDAC1 to HDAC11.)
Rabbit HDAC5 Polyclonal Antibody | anti-HDAC5 antibody
Anti-HDAC5
Reactivity
Only a weak reaction with human HDAC4 in Western Blot.HDAC5 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
HDAC5; Polyclonal Antibody; Anti-HDAC5; anti-HDAC5 antibody
Host
Rabbit
Reactivity
Only a weak reaction with human HDAC4 in Western Blot.
HDAC5 from other species may also be detectable.
HDAC5 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the human HDAC5 protein
Purity/Purification
Affinity Chromatography
Form/Format
PBS pH7.4, 50% glycerol
Applicable Applications for anti-HDAC5 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Immunogen
Synthetic peptide corresponding to c-terminus of human HDAC5 conjugated to KLH.
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot with Anti-HDAC5 (1:1000) using 25 ng of human recombinant proteins HDAC1 to HDAC11.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-HDAC5 (1:1000) using 25 ng of human recombinant proteins HDAC1 to HDAC11.)
Related Product Information for anti-HDAC5 antibody
HDAC5 or Histone deacetylase 5 belongs to the class II histone deacetylase/acuc/apha family that possesses histone deacetylase activity and represses transcription when tethered to a promoter (1). HDAC 5 plays a critical role in transcriptional regulation, cell cycle progression, and developmental events and also acts as a potential therapeutic target for the prevention of atherosclerosis. HDAC5 can co-immunoprecipitates with HDAC3 family members forming multicomplex proteins. HDAC5 can also interact with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 gene is thought to be associated with colon cancer (2).
Product Categories/Family for anti-HDAC5 antibody
References
1. Grozinger, C. M. et.al : Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc. Nat. Acad. Sci. 96: 4868-4873, 1999.
2. Scanlan, M. J. et.al: Characterization of human colon cancer antigens recognized by autologous antibodies. Int. J. Cancer 76: 652-658, 1998.
2. Scanlan, M. J. et.al: Characterization of human colon cancer antigens recognized by autologous antibodies. Int. J. Cancer 76: 652-658, 1998.
Similar Products
Product Notes
The HDAC5 (Catalog #AAA516003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HDAC5 reacts with Only a weak reaction with human HDAC4 in Western Blot. HDAC5 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's HDAC5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000. Researchers should empirically determine the suitability of the HDAC5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HDAC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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