Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Cubilin (CUBN) Polyclonal Antibody | anti-CUBN antibody
Polyclonal Antibody to Cubilin (CUBN)
MGHHHHHHSGSEF- CGGTLLGDEG IFTNPGFPDS YPNNTHCEWT IVAPSGRPVS VGFPFLSIDS SGGCDQNYLI VFNGPDANSP PFGPLCGINT GIAPFYASSN RVFIRFHAEY TTRLSGFEIM WSS
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
NCBI and Uniprot Product Information
NCBI Description
receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]
Uniprot Description
CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.
Chromosomal Location of Human Ortholog: 17q12.3
Cellular Component: apical part of cell; apical plasma membrane; brush border; brush border membrane; clathrin-coated pit; cytoplasm; endocytic vesicle; endoplasmic reticulum; endosome; endosome membrane; Golgi apparatus; lysosomal lumen; lysosomal membrane; membrane; protein complex
Molecular Function: calcium ion binding; cobalamin binding; cobalamin transporter activity; drug binding; hemoglobin binding; identical protein binding; protein binding; protein homodimerization activity; receptor activity
Biological Process: cholesterol metabolic process; cobalamin transport; hemoglobin import; in utero embryonic development; lipoprotein transport; receptor-mediated endocytosis; response to nutrient; vitamin metabolic process
Research Articles on CUBN
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Product Notes
The CUBN cubn (Catalog #AAA2003571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cubilin (CUBN) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cubilin (CUBN) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CUBN cubn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- CGGTLLGDEG IFTNPGFPDS YPNNTHCEWT IVAPSGRPVS VGFPFLSIDS SGGCDQNYLI VFNGPDANSP PFGPLCGINT GIAPFYASSN RVFIRFHAEY TTRLSGFEIM WSS. It is sometimes possible for the material contained within the vial of "Cubilin (CUBN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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