Rabbit anti-Mouse, Rat HCN3 Polyclonal Antibody | anti-HCN3 antibody

HCN3 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 3)

Cat. Num. AAA614744

• Gene Names: HCN3; KIAA1535; MGC131493
• Reactivity: Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: HCN3; Polyclonal Antibody; HCN3 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 3); Anti -HCN3 (Hyperpolarization-activated Cyclic Nucleotide-gated channel 3); anti-HCN3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes NCN3. No significant sequence homology is seen with other HCNs or any other protein. Species Sequence Homology
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid, serum, 0.1% sodium azide, 40% glycerol.

• Applicable Applications for anti-HCN3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000; ELISA: 1:10,000-1:100,000 using 50-100ng of Control Peptide/well.
• Immunogen: Synthetic peptide corresponding to 15aa mapping near the cytoplasmic, C-terminus, after the CNBD domain, of mouse/rat HCN3 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HCN3 antibody

Biological rhythms (beating of the heart, circadian sleep cycles, respiration, and the release of hormones) are necessary to sustain life. Heart beating is the most reliable and rhythmic biological phenomenon. Cardiac pacemaking is produced by the slow diastolic depolarization phase of the action potential. The hyperpolarization-activated cation current (termed If, Ih, or Iq) plays a key role in the in the initiation and modulation of cardiac and neuronal pacemaker depolarization. The generation of cardiac pacemaker potentials relies on a complex interplay between at least four different types of cation channels: T- and L-type Ca2+ channels, K+ channels, and a cation channel termed If (synonymous names are Ih and Iq). The If channel has been designated as "pacemaker" channel because it reveals unique features that are believed to be a prerequisite for pacemaker activity. Recently, the hyperpolarization-activated cyclic nucleotide-gated families of ion channel proteins (HCN1-4) have been identified as the "pacemaker" channel. The amino acid sequences of HCNs predict a structure similar to that of voltage-gated (Kv) channels and cyclic nucleotide-gated (CNG) channels. HCNs proteins (HCN1-4) are characterized by six transmembrane domains (S1-S6), including a including a positively charged voltage-sensing S4 segment and an ion-conducting pore between S5 and S6. In the C terminus the HCNs carry a cyclic nucleotide-binding domain (CNBD), a motif found in several cyclic nucleotide-binding proteins. The core region of HCNs channels (S1 to the C terminus of the CNBD) is highly conserved, whereas the cytoplasmic N and C-termini, vary considerably in their length and share only weak sequence homology. The functional HCN, like other members of the superfamily of voltage-gated cation channels, channels probably assemble into tetrameric complexes. HCN3 or HAC3 (rat 780aa, mouse 779aa, human 703aa) has been detected in brain but its expression was much less than other HCNs. Its expression was also detected in liver, lung, and kidney of mouse by some researchers but not by others.

Product Categories/Family for anti-HCN3 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 62914013
• NCBI GeneID: 57657
• UniProt Accession #: Q2T9L6; Q86WJ5; Q9P1Z3
• Molecular Weight: 15,009 Da
• NCBI Official Full Name: HCN3 protein
• NCBI Official Synonym Full Names: hyperpolarization activated cyclic nucleotide-gated potassium channel 3
• NCBI Official Symbol: HCN3
• NCBI Official Synonym Symbols: KIAA1535; MGC131493
• NCBI Protein Information: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3; OTTHUMP00000034062
• UniProt Protein Name: HCN3 protein
• Protein Family: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel
• UniProt Gene Name: HCN3
• UniProt Entry Name: Q2T9L6_HUMAN

NCBI Description

This gene encodes a multi-pass membrane protein that functions as a voltage gated cation channel. The encoded protein is a member of a family of closely related cyclic adenosine monophosphate-binding channel proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Uniprot Description

HCN3: Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Belongs to the potassium channel HCN family.

Protein type: Membrane protein, integral; Channel, cation; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: voltage-gated potassium channel activity; voltage-gated sodium channel activity; cAMP binding

Biological Process: synaptic transmission; regulation of membrane potential