Rabbit anti-Human hChk1-S280 Polyclonal Antibody | anti-hChk1-S280 antibody

hChk1-S280

Cat. Num. AAA9231347

• Gene Names: CHEK1; CHK1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: hChk1-S280; Polyclonal Antibody; Serine/threonine-protein kinase Chk1; 2.7.11.1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1; CHEK1; CHK1; anti-hChk1-S280 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 476

• Applicable Applications for anti-hChk1-S280 antibody: Western Blot (WB), ELISA (EIA)
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Dilution: WB - 1:1000Anti-hChk1-S280 at 1:1000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-hChk1-S280 antibody

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser- 124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell- cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.

Product Categories/Family for anti-hChk1-S280 antibody

Cancer

References

Sanchez Y.,et al.Science 277:1497-1501(1997). Flaggs G.,et al.Curr. Biol. 7:977-986(1997). Semba S.,et al.Int. J. Oncol. 16:731-737(2000). Pabla N.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:197-202(2012). Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 166295194
• NCBI GeneID: 1111
• NCBI Accession #: NP_001107593.1
• NCBI GenBank Nucleotide #: NM_001114121.2
• UniProt Accession #: O14757
• Molecular Weight: 54434 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk1 isoform 1
• NCBI Official Synonym Full Names: checkpoint kinase 1
• NCBI Official Symbol: CHEK1
• NCBI Official Synonym Symbols: CHK1
• NCBI Protein Information: serine/threonine-protein kinase Chk1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• UniProt Gene Name: CHEK1
• UniProt Synonym Gene Names: CHK1
• UniProt Entry Name: CHK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.

Protein type: Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; CHK1 subfamily

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: nucleoplasm; centrosome; extracellular space; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; nucleus; chromatin; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; G2/M transition of mitotic cell cycle; G2/M transition DNA damage checkpoint; DNA repair; DNA replication; response to DNA damage stimulus; regulation of cell proliferation