Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
GNAI3 recombinant protein
GNAI3, Active
Applications
Enzyme Assay
Synonyms
GNAI3; Active; 87U6; ARCND1; GNAI3 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for GNAI3 recombinant protein
Enzyme Assay
Tag
GST
Species
Human
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-Page
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for GNAI3 recombinant protein
Recombinant human GNAI3 (2-end) was expressed in E Coli cells using an N-terminal GST tag. The gene of GNAI3 accession number is NM_006496.
Guanine nucleotide-binding proteins (G proteins) are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits, and function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. G protein subunit alpha i3 (GNAI3) stimulates the activity of receptor-regulated K+ channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Guanine nucleotide-binding proteins (G proteins) are heterotrimeric signal-transducing molecules consisting of alpha, beta, and gamma subunits, and function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha subunit binds guanine nucleotide, can hydrolyze GTP, and can interact with other proteins. G protein subunit alpha i3 (GNAI3) stimulates the activity of receptor-regulated K+ channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Product Categories/Family for GNAI3 recombinant protein
References
1. Mattera R, et al: Recombinant alpha i-3 subunit of G protein activates Gk-gated K+ channels. J Biol Chem. 1989 Jan 5;264(1):465-71.
2. Cho H, et al: Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division. J Cell Biol. 2007 Jul 16;178(2):245-55.
3. http://www.uniprot.org/uniprot/P08754
2. Cho H, et al: Localization of Gi alpha proteins in the centrosomes and at the midbody: implication for their role in cell division. J Cell Biol. 2007 Jul 16;178(2):245-55.
3. http://www.uniprot.org/uniprot/P08754
NCBI and Uniprot Product Information
Similar Products
Product Notes
The GNAI3 (Catalog #AAA517024) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GNAI3 can be used in a range of immunoassay formats including, but not limited to, Enzyme Assay. Researchers should empirically determine the suitability of the GNAI3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GNAI3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
