Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: Hep G2; C: MOLT4) using a 7.5 % SDS PAGE gel and HCE antibody at a dilution of 1:1000)
Rabbit HCE Polyclonal Antibody | anti-HCE antibody
HCE antibody
Applications
Western Blot
Purity
HCE antibody was purified by antigen-affinity chromatography
Synonyms
HCE; Polyclonal Antibody; HCE antibody; Polyclonal HCE; Anti-HCE; RNA guanylyltransferase and 5'-phosphatase; RNGTT; HCE1; CAP1A; mRNA-capping enzyme; DKFZp686J2031; HCAP1; hCAP; anti-HCE antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
HCE antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
0.54 mg/ml (varies by lot)
Applicable Applications for anti-HCE antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:3000
Biological Significance
Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
Cross-Reactivity
Human
Immunogen
HCE antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 40 and 286 of HCE
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: Hep G2; C: MOLT4) using a 7.5 % SDS PAGE gel and HCE antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: HeLa; B: Hep G2; C: MOLT4) using a 7.5 % SDS PAGE gel and HCE antibody at a dilution of 1:1000)
Related Product Information for anti-HCE antibody
Affinity purified Rabbit polyclonal HCE antibody
Product Categories/Family for anti-HCE antibody
Similar Products
Product Notes
The HCE (Catalog #AAA835774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HCE can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:3000. Researchers should empirically determine the suitability of the HCE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HCE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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