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Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.)

Rabbit anti-Human, Mouse HARS Polyclonal Antibody | anti-HARS antibody

HARS Antibody

Gene Names
HARS; HRS; CMT2W; USH3B
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
HARS; Polyclonal Antibody; HARS Antibody; HRS; HisRS; histidine translase; histidine-tRNA ligase; histidyl-tRNA synthetase; anti-HARS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
HARS Antibody detects endogenous levels of total HARS protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
489
Applicable Applications for anti-HARS antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human HARS.
Immunogen Range
460-509
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.)

Immunofluorescence (IF) (Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57 kDa
NCBI Official Full Name
histidine--tRNA ligase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
histidyl-tRNA synthetase
NCBI Official Symbol
HARS
NCBI Official Synonym Symbols
HRS; CMT2W; USH3B
NCBI Protein Information
histidine--tRNA ligase, cytoplasmic
UniProt Protein Name
Histidine--tRNA ligase, cytoplasmic
Protein Family
UniProt Gene Name
HARS
UniProt Synonym Gene Names
HRS; HisRS

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Cytoplasmic histidine--tRNA ligase (Probable). Plays a role in axon guidance.

Research Articles on HARS

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Product Notes

The HARS hars (Catalog #AAA9504940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HARS Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the HARS hars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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