Human histidyl-tRNA synthetase ELISA Kit | HARS elisa kit

Human Histidyl-tRNA synthetase, cytoplasmic, HARS ELISA Kit

Cat. Num. AAA902901

• Gene Names: HARS; HRS; USH3B
• Reactivity: Human
• Synonyms: histidyl-tRNA synthetase; Human Histidyl-tRNA synthetase; cytoplasmic; HARS ELISA Kit; cytoplasmic (HARS) ELISA kit; FLJ20491; HRS; HisRS; histidine tRNA ligase 1; histidine translase; histidine-tRNA ligase; HARS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human HARS. No significant cross-reactivity or interference between human HARS and analogues was observed.
• Sequence Length: 469

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HARS elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HARS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HARS bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 384475552
• NCBI GeneID: 3035
• NCBI Accession #: NP_001244969.1
• NCBI GenBank Nucleotide #: NM_001258040.2
• UniProt Accession #: P12081; B4DHQ1; B4DY73; D6REN6; J3KNE5
• Molecular Weight: 57,411 Da
• NCBI Official Full Name: histidine--tRNA ligase, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: histidyl-tRNA synthetase
• NCBI Official Symbol: HARS
• NCBI Official Synonym Symbols: HRS; USH3B
• NCBI Protein Information: histidine--tRNA ligase, cytoplasmic; HisRS; histidine translase
• UniProt Protein Name: Histidine--tRNA ligase, cytoplasmic
• Protein Family: Histidine--tRNA ligase
• UniProt Gene Name: HARS
• UniProt Synonym Gene Names: HRS; HisRS
• UniProt Entry Name: SYHC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: EC 6.1.1.21; Translation; Ligase

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; cytosol

Molecular Function: histidine-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; histidyl-tRNA aminoacylation

Disease: Usher Syndrome, Type Iiib