Rabbit anti-Human HACD2 Polyclonal Antibody | anti-HACD2 antibody

HACD2 Antibody

Cat. Num. AAA7003184

• Gene Names: HACD2; PTPLB
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%,Protein G purified
• Synonyms: HACD2; Polyclonal Antibody; HACD2 Antibody; Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2; 3-hydroxyacyl-CoA dehydratase 21; HACD21; anti-HACD2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 254

• Applicable Applications for anti-HACD2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Recombinant human Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: HACD2 antibody at 4ug/mlLane 1: MCF7 whole cell lysateLane 2: LO2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 28 kDaObserved band size: 28 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7003184 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of MCF-7 cells using MBS7003184 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-HACD2 antibody

Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.

NCBI and Uniprot Product Information

• NCBI GI #: 38257153
• NCBI GeneID: 201562
• NCBI Accession #: NP_940684.1
• NCBI GenBank Nucleotide #: NM_198402.3
• UniProt Accession #: Q6Y1H2
• Molecular Weight: 28,368 Da
• NCBI Official Full Name: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2
• NCBI Official Synonym Full Names: 3-hydroxyacyl-CoA dehydratase 2
• NCBI Official Symbol: HACD2
• NCBI Official Synonym Symbols: PTPLB
• NCBI Protein Information: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2
• UniProt Protein Name: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2
• UniProt Gene Name: HACD2
• UniProt Synonym Gene Names: HACD2
• UniProt Entry Name: HACD2_HUMAN

Uniprot Description

PTPLB: Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Belongs to the very long-chain fatty acids dehydratase HACD family.

Protein type: EC 4.2.1.134; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q21.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane

Molecular Function: 3-hydroxyacyl-CoA dehydratase activity; enzyme binding; protein binding; protein tyrosine phosphatase activity

Biological Process: cellular lipid metabolic process; fatty acid elongation; sphingolipid biosynthetic process; triacylglycerol biosynthetic process; very-long-chain fatty acid biosynthetic process