Rabbit anti-Human GUCA1B Polyclonal Antibody | anti-GUCA1B antibody

GUCA1B, NT (GUCA1B, GCAP2, Guanylyl cyclase-activating protein 2, Guanylate cyclase activator 1B) (Biotin)

Cat. Num. AAA6305066

• Gene Names: GUCA1B; RP48; GCAP2; GUCA2; DKFZp686E1183
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GUCA1B; Polyclonal Antibody; NT (GUCA1B; GCAP2; Guanylyl cyclase-activating protein 2; Guanylate cyclase activator 1B) (Biotin); Guanylate cyclase activator 1B; anti-GUCA1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GUCA1B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: GUCA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human GUCA1B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GUCA1B Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GUCA1B antibody detected the GUCA1B protein (arrow).)

Related Product Information for anti-GUCA1B antibody

The protein encoded by this gene is a calcium-binding protein that activates photoreceptor guanylate cyclases. This gene may have arisen due to a gene duplication event since there is a highly similar gene clustered with it on chromosome 6. Mutations in this gene can cause a form of retinitis pigmentosa. [provided by RefSeq].

Product Categories/Family for anti-GUCA1B antibody

Antibodies; Enzymes; Cyclase

References

Sato, M., et al. Graefes Arch. Clin. Exp. Ophthalmol. 243(3):235-242(2005). Nishiguchi, K.M., et al. Invest. Ophthalmol. Vis. Sci. 45(11):3863-3870(2004). Mungall, A.J., et al. Nature 425(6960):805-811(2003). Wistow, G., et al. Mol. Vis. 8, 196-204 (2002): Zhang, Q.J., et al. Yi Chuan 24(1):19-21(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 153945858
• NCBI GeneID: 2979
• NCBI Accession #: NP_002089.4
• NCBI GenBank Nucleotide #: NM_002098.5
• UniProt Accession #: Q9UMX6; Q7Z3V9
• Molecular Weight: 23,318 Da
• NCBI Official Full Name: guanylyl cyclase-activating protein 2
• NCBI Official Synonym Full Names: guanylate cyclase activator 1B (retina)
• NCBI Official Symbol: GUCA1B
• NCBI Official Synonym Symbols: RP48; GCAP2; GUCA2; DKFZp686E1183
• NCBI Protein Information: guanylyl cyclase-activating protein 2; GCAP 2; OTTHUMP00000016398; guanylate cyclase-activating protein, photoreceptor 2
• UniProt Protein Name: Guanylyl cyclase-activating protein 2
• Protein Family: Guanylyl cyclase-activating protein
• UniProt Gene Name: GUCA1B
• UniProt Synonym Gene Names: GCAP2; GCAP 2
• UniProt Entry Name: GUC1B_HUMAN

Uniprot Description

GCAP2: Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure. Defects in GUCA1B are the cause of retinitis pigmentosa type 48 (RP48). RP48 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: photoreceptor inner segment

Molecular Function: calcium sensitive guanylate cyclase activator activity; calcium ion binding

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; cell-cell signaling; receptor guanylyl cyclase signaling pathway; positive regulation of guanylate cyclase activity; body fluid secretion

Disease: Retinitis Pigmentosa 48