Western Blot (WB)
(Western blot analysis of HSD17B12 using A549 whole cell lysates)
Rabbit anti-Human HSD17B12 Polyclonal Antibody | anti-HSD17B12 antibody
HSD17B12 Antibody
Gene Names
HSD17B12; KAR; SDR12C1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HSD17B12; Polyclonal Antibody; HSD17B12 Antibody; 12; 3-ketoacyl-CoA reductase; short chain; 17 beta HSD 12; 17 beta hydroxysteroid dehydrogenase 12; 17-beta-HSD XII; 17-beta-hydroxysteroid dehydrogenase XII; 17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; 17beta HSD type 12; 3 ketoacyl CoA reductase; DHB12_HUMAN; Estradiol 17 beta dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; Hydroxysteroid (17-beta) dehydrogenase 12; KAR; KIK-I; Kik1; SDR12C1; Short chain dehydrogenase/reductase family 12C member 1; Steroid dehydrogenase homolog; anti-HSD17B12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
HSD17B12 antibody detects endogenous levels of total HSD17B12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
312
Applicable Applications for anti-HSD17B12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human HSD17B12
Subcellular Location
Endoplasmic Reticulum Membrane.
Tissue Specificity
Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of HSD17B12 using A549 whole cell lysates)
Western Blot (WB)
(Western blot analysis of HSD17B12 using A549 whole cell lysates)
Related Product Information for anti-HSD17B12 antibody
Function: Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long-and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
Subunit Structure: Interacts with ELOVL1 and LASS2.
Similarity: The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
Subunit Structure: Interacts with ELOVL1 and LASS2.
Similarity: The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 34 kDa
Predicted: 35 kDa
Predicted: 35 kDa
NCBI Official Full Name
very-long-chain 3-oxoacyl-CoA reductase
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 12
NCBI Official Symbol
HSD17B12
NCBI Official Synonym Symbols
KAR; SDR12C1
NCBI Protein Information
very-long-chain 3-oxoacyl-CoA reductase
UniProt Protein Name
Very-long-chain 3-oxoacyl-CoA reductase
Protein Family
UniProt Gene Name
HSD17B12
UniProt Synonym Gene Names
17-beta-HSD 121 PublicationManual assertion based on opinion iniRef.10; KAR1 PublicationManual assertion based on opinion iniRef.8
NCBI Description
This gene encodes a very important 17beta-hydroxysteroid dehydrogenase (17beta-HSD) that converts estrone into estradiol in ovarian tissue. This enzyme is also involved in fatty acid elongation. [provided by RefSeq, Oct 2011]
Uniprot Description
Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
Research Articles on HSD17B12
Similar Products
Product Notes
The HSD17B12 hsd17b12 (Catalog #AAA9609822) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD17B12 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the HSD17B12 hsd17b12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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