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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GTF3C4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Rabbit anti-Human GTF3C4 Polyclonal Antibody | anti-GTF3C4 antibody

GTF3C4 Polyclonal Antibody

Gene Names
GTF3C4; KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Affinity purification
Synonyms
GTF3C4; Polyclonal Antibody; GTF3C4 Polyclonal Antibody; KAT12; TFIII90; TFIIIC90; TFIIIC290; TFIIICDELTA; anti-GTF3C4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
822
Applicable Applications for anti-GTF3C4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500 - 1:2000, IP: 1:20 - 1:50
Immunogen
Recombinant protein of human GTF3C4
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GTF3C4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GTF3C4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
Related Product Information for anti-GTF3C4 antibody
GTF3C4 (general transcription factor IIIC, polypeptide 4, 90kDa) is a protein-coding gene. GO annotations related to this gene include histone acetyltransferase activity and enzyme activator activity.
Product Categories/Family for anti-GTF3C4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 92kDa
Molecular Weight: 822
NCBI Official Full Name
general transcription factor 3C polypeptide 4
NCBI Official Synonym Full Names
general transcription factor IIIC subunit 4
NCBI Official Symbol
GTF3C4
NCBI Official Synonym Symbols
KAT12; TFIII90; TFIIIC90; TFIIIC290; TF3C-delta; TFIIICDELTA
NCBI Protein Information
general transcription factor 3C polypeptide 4
UniProt Protein Name
General transcription factor 3C polypeptide 4
UniProt Gene Name
GTF3C4
UniProt Synonym Gene Names
TFIIIC 90 kDa subunit; TFIIIC90
UniProt Entry Name
TF3C4_HUMAN

Uniprot Description

GTF3C4: Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box. Belongs to the TFIIIC subunit 4 family.

Protein type: Acetyltransferase; EC 2.3.1.48

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: nucleoplasm; transcription factor TFIIIC complex

Molecular Function: DNA binding; enzyme activator activity; histone acetyltransferase activity; protein binding

Biological Process: 5S class rRNA transcription; gene expression; histone acetylation; positive regulation of catalytic activity; transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; transcription, DNA-dependent; tRNA transcription from RNA polymerase III promoter

Research Articles on GTF3C4

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Product Notes

The GTF3C4 gtf3c4 (Catalog #AAA9129285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF3C4 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GTF3C4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500 - 1:2000, IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the GTF3C4 gtf3c4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF3C4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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