Western Blot (WB)
(Western blot analysis of GTF2E2 (arrow) using rabbit polyclonal GTF2E2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.)
Rabbit GTF2E2 Polyclonal Antibody | anti-GTF2E2 antibody
GTF2E2 Antibody (C-term)
Gene Names
GTF2E2; FE; TF2E2; TFIIE-B
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GTF2E2; Polyclonal Antibody; GTF2E2 Antibody (C-term); Transcription initiation factor IIE subunit beta; TFIIE-beta; General transcription factor IIE subunit 2; TF2E2; anti-GTF2E2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GTF2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the C-terminal region of human GTF2E2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
240-269
Sequence Length
291
Applicable Applications for anti-GTF2E2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of GTF2E2 (arrow) using rabbit polyclonal GTF2E2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.)
Western Blot (WB)
(Western blot analysis of GTF2E2 (arrow) using rabbit polyclonal GTF2E2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.)
Western Blot (WB)
(GTF2E2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GTF2E2 antibody detected the GTF2E2 protein (arrow).)
Western Blot (WB)
(GTF2E2 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GTF2E2 antibody detected the GTF2E2 protein (arrow).)
Related Product Information for anti-GTF2E2 antibody
GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
References
Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010)
Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010)
Tanaka, A., et al. Genes Cells 14(3):395-405(2009)
Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Watanabe, T., et al. Mol. Cell. Biol. 23(8):2914-2926(2003)
Festen, E.A., et al. Am. J. Gastroenterol. 105(2):395-402(2010)
Tanaka, A., et al. Genes Cells 14(3):395-405(2009)
Jawhari, A., et al. EMBO Rep. 7(5):500-505(2006)
Watanabe, T., et al. Mol. Cell. Biol. 23(8):2914-2926(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33044
NCBI Official Full Name
transcription initiation factor IIE subunit beta
NCBI Official Synonym Full Names
general transcription factor IIE, polypeptide 2, beta 34kDa
NCBI Official Symbol
GTF2E2
NCBI Official Synonym Symbols
FE; TF2E2; TFIIE-B
NCBI Protein Information
transcription initiation factor IIE subunit beta
UniProt Protein Name
Transcription initiation factor IIE subunit beta
Protein Family
UniProt Gene Name
GTF2E2
UniProt Synonym Gene Names
TF2E2; TFIIE-beta
UniProt Entry Name
T2EB_HUMAN
Uniprot Description
GTF2E2: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Belongs to the TFIIE beta subunit family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 8p12
Cellular Component: nucleoplasm; cytoplasm; nucleus; transcription factor TFIIE complex
Molecular Function: protein binding; DNA binding
Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; viral reproduction; RNA elongation from RNA polymerase II promoter; gene expression
Research Articles on GTF2E2
Similar Products
Product Notes
The GTF2E2 gtf2e2 (Catalog #AAA9204580) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 240-269. The GTF2E2 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2E2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the GTF2E2 gtf2e2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GTF2E2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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