Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GTF2E1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5S.)
Rabbit anti-Human, Mouse GTF2E1 Polyclonal Antibody | anti-GTF2E1 antibody
GTF2E1 Polyclonal Antibody
Gene Names
GTF2E1; FE; TF2E1; TFIIE-A
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity Purification
Synonyms
GTF2E1; Polyclonal Antibody; GTF2E1 Polyclonal Antibody; FE; TF2E1; TFIIE-A; anti-GTF2E1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
LAGGHHREAWATKGPSYEDLYTQNVVINMDDQEDLHRASLEGKSAKERPIWLRESTVQGAYGSEDMKEGGIDMDAFQEREEGHAGPDDNEEVMRALLIHEKKTSSAMAGSVGAAAPVTAANGSDSESETSESDDDSPPRPAAVAVHKREEDEEEDDEFEEVADDPIVMVAGRPFSYSEVSQRPELVAQMTPEEKEAYIAMGQRMFEDLFE
Sequence Length
439
Applicable Applications for anti-GTF2E1 antibody
Western Blot (WB)
Application Notes
WB: 1:200 - 1:2000
Immunogen
Recombinant protein of human GTF2E1
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Nucleus
Positive Samples
HeLa, Jurkat, A-431, Mouse brain, Mouse spleen, Mouse testis
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GTF2E1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5S.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GTF2E1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5S.)
Product Categories/Family for anti-GTF2E1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 49kDa
Observed: 60kDa
Observed: 60kDa
NCBI Official Full Name
general transcription factor IIE subunit 1
NCBI Official Synonym Full Names
general transcription factor IIE subunit 1
NCBI Official Symbol
GTF2E1
NCBI Official Synonym Symbols
FE; TF2E1; TFIIE-A
NCBI Protein Information
general transcription factor IIE subunit 1
UniProt Protein Name
General transcription factor IIE subunit 1
Protein Family
UniProt Gene Name
GTF2E1
UniProt Synonym Gene Names
TF2E1; TFIIE-alpha
Uniprot Description
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Research Articles on GTF2E1
Similar Products
Product Notes
The GTF2E1 gtf2e1 (Catalog #AAA9135261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GTF2E1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GTF2E1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200 - 1:2000. Researchers should empirically determine the suitability of the GTF2E1 gtf2e1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LAGGHHREAW ATKGPSYEDL YTQNVVINMD DQEDLHRASL EGKSAKERPI WLRESTVQGA YGSEDMKEGG IDMDAFQERE EGHAGPDDNE EVMRALLIHE KKTSSAMAGS VGAAAPVTAA NGSDSESETS ESDDDSPPRP AAVAVHKREE DEEEDDEFEE VADDPIVMVA GRPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE. It is sometimes possible for the material contained within the vial of "GTF2E1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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