Rabbit anti-Human GTSE1 Polyclonal Antibody | anti-GTSE1 antibody

GTSE1, CT (GTSE1, G2 and S phase-expressed protein 1, Protein B99 homolog) (PE)

Cat. Num. AAA6305052

• Gene Names: GTSE1; B99
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GTSE1; Polyclonal Antibody; CT (GTSE1; G2 and S phase-expressed protein 1; Protein B99 homolog) (PE); Protein B99 homolog; anti-GTSE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-GTSE1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GTSE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 691~720 amino acids from the C-terminal region of human GTSE1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GTSE1 Antibody (C-term) in Hela cell line lysates (35ug/lane). GTSE1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GTSE1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GTSE1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GTSE1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GTSE1 antibody

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis.

Product Categories/Family for anti-GTSE1 antibody

Antibodies; Enzymes

References

Bublik, D.R., et al. J. Biol. Chem. 285(8):5274-5281(2010). Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006). Kapranov, P., et al. Genome Res. 15(7):987-997(2005). Cheng, J., et al. Science 308(5725):1149-1154(2005). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 317373439
• NCBI GeneID: 51512
• UniProt Accession #: Q9NYZ3; Q20WK2; Q53GX5; Q5R3I6; Q6DHX4; Q9BRE0; Q9UGZ9; Q9Y557; B0QYM3
• Molecular Weight: 720
• NCBI Official Full Name: G2 and S phase-expressed protein 1
• NCBI Official Synonym Full Names: G-2 and S-phase expressed 1
• NCBI Official Symbol: GTSE1
• NCBI Official Synonym Symbols: B99
• NCBI Protein Information: G2 and S phase-expressed protein 1; protein B99 homolog
• UniProt Protein Name: G2 and S phase-expressed protein 1
• Protein Family: G2 and S phase-expressed protein
• UniProt Gene Name: GTSE1
• UniProt Synonym Gene Names: GTSE-1
• UniProt Entry Name: GTSE1_HUMAN

NCBI Description

The protein encoded by this gene is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shuttling it out of the nucleus and repressing its ability to induce apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

GTSE1: May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression.

Protein type: Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q13.2-q13.3

Cellular Component: membrane; cytoplasmic microtubule

Molecular Function: protein binding

Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; microtubule-based process