Rabbit anti-Human, Mouse GSTK1 Polyclonal Antibody | anti-GSTK1 antibody

GSTK1, ID (GSTK1, Glutathione S-transferase kappa 1, GST 13-13, GST class-kappa, GSTK1-1, Glutathione S-transferase subunit 13) (FITC)

Cat. Num. AAA6304726

• Gene Names: GSTK1; GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GSTK1; Polyclonal Antibody; ID (GSTK1; Glutathione S-transferase kappa 1; GST 13-13; GST class-kappa; GSTK1-1; Glutathione S-transferase subunit 13) (FITC); Glutathione S-transferase subunit 13; anti-GSTK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-GSTK1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GSTK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99~128 amino acids from the Central region of human GSTK1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of GSTK1 Antibody (Center) in mouse liver tissue lysates (35ug/lane). GSTK1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(GSTK1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSTK1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GSTK1 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GSTK1 antibody

GSTK1 encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-GSTK1 antibody

Antibodies; GST

References

Santos, G.S., et al. Int. J. Mol. Med. 24(3):393-399(2009). Aydos, S.E., et al. Fertil. Steril. 92(2):541-547(2009). Gao, F., et al. Endocr. J. 56(3):487-494(2009). Katoh, T., et al. Pharmacogenomics 9(1):93-104(2008). Li, J., et al. Protein Sci. 14(9):2361-2369(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 12643338
• NCBI GeneID: 373156
• UniProt Accession #: Q9Y2Q3; Q6P4H0; Q7Z520; Q9P1S4; B4DIH1; B4DSY2
• Molecular Weight: 226
• NCBI Official Full Name: Glutathione S-transferase kappa 1
• NCBI Official Synonym Full Names: glutathione S-transferase kappa 1
• NCBI Official Symbol: GSTK1
• NCBI Official Synonym Symbols: GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13
• NCBI Protein Information: glutathione S-transferase kappa 1; GST class-kappa; glutathione S-transferase k1; glutathione S-transferase subunit 13 homolog
• UniProt Protein Name: Glutathione S-transferase kappa 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTK1
• UniProt Synonym Gene Names: hGSTK1
• UniProt Entry Name: GSTK1_HUMAN

NCBI Description

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Uniprot Description

GSTK1: Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). Belongs to the GST superfamily. Kappa family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Mitochondrial; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: membrane; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrial inner membrane; peroxisome; intracellular

Molecular Function: glutathione transferase activity; protein disulfide oxidoreductase activity; glutathione peroxidase activity; receptor binding

Biological Process: glutathione metabolic process; epithelial cell differentiation