GSTK1 recombinant protein

GSTK1 Protein, Human, Recombinant

Cat. Num. AAA8121659

• Gene Names: GSTK1; GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GSTK1; GSTK1 Protein; Human; Recombinant; Human GSTK1 Protein; GST Protein; GST13 Protein; GST13-13 Protein; GSTK1-1 Protein; hGSTK1 Protein; glutathione S-transferase kappa 1; GSTK1 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris,10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly2-Leu226

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human GSTK1 (Q9Y2Q3-1)(Gly2-Leu226) was expressed with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for GSTK1 recombinant protein

Background: GSTK1 gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. Glutathione S-transferases (GSTs) are a family of enzymes that catalyze a variety of reactions in both eukaryotes and prokaryotes. They catalyze the conjugation of reduced glutathione with potentially toxic, xenobiotic substrates, thus aiding excretion from the body. GSTK1(glutathione S-transferase kappa 1) is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. GSTK1 functions in cellular detoxification.

References

Zhang QH, et al. (2001) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10(10):1546-60. Morel F, et al. (2004) Gene and protein characterization of the human glutathione S-transferase kappa and evidence for a peroxisomal localization. J Biol Chem. 279(16): 16246-53. Jowsey IR, et al. (2003) Biochemical and genetic characterization of a murine class Kappa glutathione S-transferase. Biochem J. 373(Pt 2):559-69.

NCBI and Uniprot Product Information

• NCBI GI #: 12643338
• NCBI GeneID: 373156
• UniProt Accession #: Q9Y2Q3; Q6P4H0; Q7Z520; Q9P1S4; B4DIH1; B4DSY2
• Molecular Weight: 226
• NCBI Official Full Name: Glutathione S-transferase kappa 1
• NCBI Official Synonym Full Names: glutathione S-transferase kappa 1
• NCBI Official Symbol: GSTK1
• NCBI Official Synonym Symbols: GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13
• NCBI Protein Information: glutathione S-transferase kappa 1; GST class-kappa; glutathione S-transferase k1; glutathione S-transferase subunit 13 homolog
• UniProt Protein Name: Glutathione S-transferase kappa 1
• Protein Family: Glutathione S-transferase
• UniProt Gene Name: GSTK1
• UniProt Synonym Gene Names: hGSTK1
• UniProt Entry Name: GSTK1_HUMAN

NCBI Description

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

Uniprot Description

GSTK1: Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). Belongs to the GST superfamily. Kappa family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; Mitochondrial; Xenobiotic Metabolism - drug metabolism - cytochrome P450

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: membrane; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrial inner membrane; peroxisome; intracellular

Molecular Function: glutathione transferase activity; protein disulfide oxidoreductase activity; glutathione peroxidase activity; receptor binding

Biological Process: glutathione metabolic process; epithelial cell differentiation