Western Blot (WB)
(Western blot analysis of GSPT1 expression in Hela (A), HepG2 (B), NIH3T3 (C) whole cell lysates.)
Rabbit GSPT1 Polyclonal Antibody | anti-GSPT1 antibody
Anti-GSPT1 Antibody
Gene Names
GSPT1; GST1; ETF3A; eRF3a; 551G9.2
Reactivity
Human, Mouse, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
GSPT1; Polyclonal Antibody; Anti-GSPT1 Antibody; ERF3A; Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog; anti-GSPT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Zebrafish
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of GSPT1 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Sequence Length
499
Applicable Applications for anti-GSPT1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSPT1. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of GSPT1 expression in Hela (A), HepG2 (B), NIH3T3 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GSPT1 expression in Hela (A), HepG2 (B), NIH3T3 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GSPT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GSPT1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-GSPT1 antibody
Rabbit polyclonal antibody to GSPT1
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,700 Da
NCBI Official Full Name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
NCBI Official Synonym Full Names
G1 to S phase transition 1
NCBI Official Symbol
GSPT1
NCBI Official Synonym Symbols
GST1; ETF3A; eRF3a; 551G9.2
NCBI Protein Information
eukaryotic peptide chain release factor GTP-binding subunit ERF3A
UniProt Protein Name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Protein Family
UniProt Gene Name
GSPT1
UniProt Synonym Gene Names
ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a
UniProt Entry Name
ERF3A_HUMAN
Uniprot Description
Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ERF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.
Research Articles on GSPT1
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Product Notes
The GSPT1 gspt1 (Catalog #AAA8243160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GSPT1 Antibody reacts with Human, Mouse, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's GSPT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200). Researchers should empirically determine the suitability of the GSPT1 gspt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSPT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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