Human esRAGE (Endogenous secretory Receptor for Advanced Glycation End product) ELISA Kit | esRAGE elisa kit

Human esRAGE (Endogenous secretory Receptor for Advanced Glycation End product)

Cat. Num. AAA766094

• Gene Names: Ager; RAGE
• Reactivity: Human
• Synonyms: esRAGE (Endogenous secretory Receptor for Advanced Glycation End product); Human esRAGE (Endogenous secretory Receptor for Advanced Glycation End product); esRAGE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of esRAGE. No significant cross-reactivity or interference between esRAGE and analogues was observed.
• Sequence Length: 334

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: 4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for esRAGE elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 84570471
• NCBI GeneID: 11596
• Molecular Weight: 3,036 Da
• NCBI Official Full Name: endogenous secretory receptor for advanced glycation endproducts
• NCBI Official Synonym Full Names: advanced glycosylation end product-specific receptor
• NCBI Official Symbol: Ager
• NCBI Official Synonym Symbols: RAGE
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: Ager
• UniProt Synonym Gene Names: Rage
• UniProt Entry Name: RAGE_MOUSE

Uniprot Description

RAGE: Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF- alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Interacts with S100B, S100A1 and APP. Interacts with S100A12. Endothelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Cell cycle regulation; Membrane protein, integral

Cellular Component: axon; basal plasma membrane; cell soma; cell surface; cytoplasm; cytosol; external side of plasma membrane; extracellular region; extracellular space; integral to membrane; membrane; nucleus; plasma membrane

Molecular Function: advanced glycation end-product receptor activity; heparin binding; identical protein binding; protein binding

Biological Process: activation of NF-kappaB transcription factor; astrocyte development; calcium ion homeostasis; induction of positive chemotaxis; inflammatory response; JAK-STAT cascade; negative regulation of cell adhesion; negative regulation of cell migration; negative regulation of collagen biosynthetic process; negative regulation of endothelial cell proliferation; negative regulation of interleukin-10 production; negative regulation of protein amino acid phosphorylation; neurite development; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of cell migration; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interleukin-12 production; positive regulation of JNK activity; positive regulation of neuron apoptosis; positive regulation of phagocytosis, engulfment; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; regulation of DNA binding; regulation of inflammatory response; regulation of T cell mediated cytotoxicity; response to hypoxia