Western Blot (WB)
(Western blot analysis of GSK3 alpha expression in Jurkat (A), HeLa (B), Raw264.7 (C), PC12 (D) whole cell lysates.)
Rabbit GSK3 alpha Polyclonal Antibody | anti-GSK3A antibody
Anti-GSK3 alpha Antibody
Western Blot (WB)
(Western blot analysis of GSK3 alpha expression in Jurkat (A), HeLa (B), Raw264.7 (C), PC12 (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of GSK3 alpha expression in Jurkat (A), HeLa (B), Raw264.7 (C), PC12 (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GSK3 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of GSK3 alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of GSK3 alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of GSK3 alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]
Uniprot Description
GSK3A: a proline-directed protein kinase of the GSK family. Implicated in the control of several regulatory proteins including glycogen synthase, Myb, and c-Jun. GSK3 and GSK3 have similar functions. GSK3 phophorylates tau, the principal component of neurofibrillary tangles in Alzheimer disease and is required for maximal production of amyloid plaque peptides by secretase. A GSK3 promoter SNP effects progression of bipolar disorder. The GSK3 inhibitor, lithium, is used to treat bipolar disorder and is seen to block plaque formation. GSK3 generally opposes the action of insulin, and GSK3 hyperactivity is thought to contribute to insulin resistant (type II) diabetes. GSK3 also negatively regulates cardiac hypertrophy. A tumor suppressor role is indicated by the oncogenic potential of stabilized -catenin mutants that lack GSK3 phosphorylation sites. Inhibitor: AR-A014418
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.26; EC 2.7.11.1; CMGC group; GSK family; GSK subfamily
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: beta-catenin destruction complex; cytosol; mitochondrion
Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity; tau-protein kinase activity
Biological Process: aging; cellular protein metabolic process; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glycogen metabolic process; innate immune response; insulin receptor signaling pathway; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of TOR signaling pathway; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of cAMP biosynthetic process; positive regulation of heart contraction; positive regulation of neuron apoptosis; positive regulation of protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid phosphorylation; regulation of systemic arterial blood pressure; unfolded protein response; Wnt receptor signaling pathway
Research Articles on GSK3A
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Product Notes
The GSK3A gsk3a (Catalog #AAA8241917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-GSK3 alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GSK3 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500); Immunoprecipitation: (1/10 - 1/100). Researchers should empirically determine the suitability of the GSK3A gsk3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GSK3 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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