Mouse GSK3 alpha Monoclonal Antibody | anti-GSK3 alpha antibody

GSK3 alpha antibody

Cat. Num. AAA5310885

• Applications: ELISA, Immunofluorescence, Western Blot
• Synonyms: GSK3 alpha; Monoclonal Antibody; GSK3 alpha antibody; Mouse monoclonal GSK3 alpha antibody; Monoclonal GSK3 alpha antibody; Anti-GSK3 alpha antibody; GSK-3 alpha antibody; GSK 3 alpha antibody; Glycogen synthase kinase 3 alpha antibody; anti-GSK3 alpha antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9D5G1
• Form/Format: Liquid in ascitic fluid containing 0.03% sodium azide.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-GSK3 alpha antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: ELISA: 1:10,000; IF: 1:200-1:1000; WB: 1:500-1:2000
• Immunogen: GSK3 alpha antibody was raised in Mouse using a purified recombinant fragment of GSK3 alpha expressed in E Coli as the immunogen.
• Cross-Reactivity: Human
• Preparation and Storage: Store at 4 degree C for short term storage.; Store at-20 degree C for long term storage.; Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells using GSK3 alpha antibody showing cytoplasmic localization.)

Western Blot (WB)

(Western Blot showing GSK3 alpha antibody used against Hela (1) and PC-3 cell lysate.)

Related Product Information for anti-GSK3 alpha antibody

Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

Product Categories/Family for anti-GSK3 alpha antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 49574532
• NCBI GeneID: 2931
• NCBI Accession #: NP_063937.2
• NCBI GenBank Nucleotide #: NM_019884.2
• UniProt Accession #: P49840; O14959
• Molecular Weight: 50,981 Da
• NCBI Official Full Name: glycogen synthase kinase-3 alpha
• NCBI Official Synonym Full Names: glycogen synthase kinase 3 alpha
• NCBI Official Symbol: GSK3A
• NCBI Protein Information: glycogen synthase kinase-3 alpha
• UniProt Protein Name: Glycogen synthase kinase-3 alpha
• UniProt Gene Name: GSK3A
• UniProt Synonym Gene Names: GSK-3 alpha

NCBI Description

This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011]

Uniprot Description

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.