Testing Data
(Human CD4+CD25+ cells were stained with MBS602276 (filled histogram) or isotype control (open histogram). Splenocytes were costained with APC-conjugated anti-mouse CD25, and quad marker was set based on isotype control staining.)
Mouse anti-Human, Mouse Latency Associated Peptide, Transforming Growth Factor beta1 Monoclonal Antibody
Latency Associated Peptide, Transforming Growth Factor beta1 (LAP, TGFb1) (PE)
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Latency Associated Peptide; Transforming Growth Factor beta1; Monoclonal Antibody; Transforming Growth Factor beta1 (LAP; TGFb1) (PE); Anti -Latency Associated Peptide; anti-Latency Associated Peptide; Transforming Growth Factor beta1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H66
Specificity
Recognizes human LAP (TGFb1). Species Crossreactivity: Mouse
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-Latency Associated Peptide, Transforming Growth Factor beta1 antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using CD4+CD25+ human PBMCs and mouse splenocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human or mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking add 10ul of L1393-30 to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human or Mouse Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody.
Dilution: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using CD4+CD25+ human PBMCs and mouse splenocytes.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human or mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking add 10ul of L1393-30 to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer . Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human or Mouse Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody.
Immunogen
Recombinant corresponding to aa30-278 of human LAP (TGF-b1) expressed in S. frugiperda insect ovarian cell line Sf 21 (P01137).
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at least 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Testing Data
(Human CD4+CD25+ cells were stained with MBS602276 (filled histogram) or isotype control (open histogram). Splenocytes were costained with APC-conjugated anti-mouse CD25, and quad marker was set based on isotype control staining.)
Testing Data
(Human CD4+CD25+ cells were stained with MBS602276 (filled histogram) or isotype control (open histogram). Splenocytes were costained with APC-conjugated anti-mouse CD25, and quad marker was set based on isotype control staining.)
Testing Data
(Mouse splenocytes were stained with MBS602276 (filled histogram) or isotype control (open histogram). Splenocytes were costained with APC-conjugated anti-mouse CD25, and quad marker was set based on isotype control staining.)
Testing Data
(Mouse splenocytes were stained with MBS602276 (filled histogram) or isotype control (open histogram). Splenocytes were costained with APC-conjugated anti-mouse CD25, and quad marker was set based on isotype control staining.)
Related Product Information for anti-Latency Associated Peptide, Transforming Growth Factor beta1 antibody
TGF-beta is secreted by cells in the form of an inactive complex. This complex consists of TGF-beta associated non-covalently with a protein designated the latency associated peptide (LAP). TGF-beta and LAP represent components of a pro-peptide that is cleaved in a post-golgi compartment prior to secretion. LAP and TGF-beta each consist of a disulfide-linked homodimer and the association of these two components renders TGF-beta inactive and inaccessible to anti-TGF-beta antibodies.
Product Categories/Family for anti-Latency Associated Peptide, Transforming Growth Factor beta1 antibody
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Product Notes
The Latency Associated Peptide, Transforming Growth Factor beta1 (Catalog #AAA602276) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Latency Associated Peptide, Transforming Growth Factor beta1 (LAP, TGFb1) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Latency Associated Peptide, Transforming Growth Factor beta1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 10ul labels 1x10e6 cells. Validated using CD4+CD25+ human PBMCs and mouse splenocytes. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human or mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2. After blocking add 10ul of L1393-30 to 1x10e6 cells and incubate for 30 minutes at RT. 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human or Mouse Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG1 antibody. Researchers should empirically determine the suitability of the Latency Associated Peptide, Transforming Growth Factor beta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Latency Associated Peptide, Transforming Growth Factor beta1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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