Rabbit anti-Human GRP94 Polyclonal Antibody | anti-HSP90B1 antibody

GRP94, CT (Endoplasmin, 94kD Glucose-regulated Protein, GRP-94, Heat Shock Protein 90kD beta Member 1, Tumor Rejection Antigen 1, gp96 Homolog, HSP90B1, TRA1)

Cat. Num. AAA6004234

• Gene Names: HSP90B1; TRA1; GRP94
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GRP94; Polyclonal Antibody; CT (Endoplasmin; 94kD Glucose-regulated Protein; GRP-94; Heat Shock Protein 90kD beta Member 1; Tumor Rejection Antigen 1; gp96 Homolog; HSP90B1; TRA1); Anti -GRP94; anti-HSP90B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human GRP94.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS, pH 7.4, BSA, sodium azide.

• Applicable Applications for anti-HSP90B1 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to the C-terminal of human GRP94.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Human breast ca. stained with GRP94)

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with HSP90B1 antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-HSP90B1 antibody

GRP94, also known as Heat shock protein 90kDa beta, member 1 (HSP90B1), is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. It plays an important role in maintaining protein homeostasis in the secretory pathway. Also, GRP94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. Recombinant human GRP94 protein, fused to His-tag at N-terminus.

Product Categories/Family for anti-HSP90B1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 403497
• NCBI GeneID: 404019
• UniProt Accession #: P41148
• Molecular Weight: 92,514 Da
• NCBI Official Full Name: GRP94
• NCBI Official Symbol: HSP90B1
• NCBI Official Synonym Symbols: TRA1; GRP94
• NCBI Protein Information: endoplasmin; GRP-94; tumor rejection antigen 1; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; heat shock protein 90 kDa beta member 1
• UniProt Protein Name: Endoplasmin
• Protein Family: Endoplasmin
• UniProt Gene Name: HSP90B1
• UniProt Synonym Gene Names: GRP94; TRA1; GRP-94
• UniProt Entry Name: ENPL_CANFA

Uniprot Description

GRP94: Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity. Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP. Interacts with TLR4 and TLR9, but not with TLR3. Belongs to the heat shock protein 90 family.

Protein type: Secreted, signal peptide; Apoptosis; Secreted; Heat shock protein

Cellular Component: extracellular matrix; endoplasmic reticulum membrane; focal adhesion; sarcoplasmic reticulum; endoplasmic reticulum lumen; perinuclear region of cytoplasm; melanosome; plasma membrane; midbody; nucleus; cytosol

Molecular Function: low-density lipoprotein receptor binding; RNA binding; unfolded protein binding; virion binding; protein phosphatase binding; ATP binding

Biological Process: actin rod formation; ER-associated protein catabolic process; protein folding; response to hypoxia; regulation of phosphoprotein phosphatase activity; negative regulation of apoptosis