Rabbit Growth Associated Protein 43 Polyclonal Antibody | anti-GAP43 antibody

Growth Associated Protein 43, phosphorylated (Ser41) (GAP43, Neuromodulin, PP46, p57, B-50, F1, Axonal membrane protein GAP-43, Growth-associated protein 43, Neural phosphoprotein B-50)

Cat. Num. AAA625026

• Gene Names: GAP43; B-50; PP46
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Growth Associated Protein 43; Polyclonal Antibody; phosphorylated (Ser41) (GAP43; Neuromodulin; PP46; p57; B-50; F1; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50); Anti -Growth Associated Protein 43; anti-GAP43 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Specific for Growth Associated Peptide 43 (GAP-43), when phosphorylated at serine 41.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.

• Applicable Applications for anti-GAP43 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry (frozen).; Dilution: Western Blot:1:1000, detects a band of approximately 50kD in rat brain lysates.; Optimal dilution determined by the researcher.
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within GAP-43 which includes phosphorylated Ser 41.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot of rat cortex lysate showing phosphospecific immunolabeling of the ~50kD GAP-43 protein phosphorylated at Ser41 using MBS625026.)

Related Product Information for anti-GAP43 antibody

GAP-43 is expressed in neuronal growth cones during development and during axonal regeneration.

Product Categories/Family for anti-GAP43 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 119600000
• NCBI GeneID: 2596
• UniProt Accession #: P17677; Q5U058; Q6LB24
• Molecular Weight: 24,803 Da
• NCBI Official Full Name: growth associated protein 43
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; OTTHUMP00000165225; OTTHUMP00000165226; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; postsynaptic density; cytoplasm; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: glial cell differentiation; cell fate commitment; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation