Rabbit anti-Human APPL2 Polyclonal Antibody | anti-APPL2 antibody

APPL2, NT (APPL2, DIP13B, DCC-interacting protein 13-beta, Adapter protein containing PH domain, PTB domain and leucine zipper motif 2) (Biotin)

Cat. Num. AAA6274590

• Gene Names: APPL2; DIP13B
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: APPL2; Polyclonal Antibody; NT (APPL2; DIP13B; DCC-interacting protein 13-beta; Adapter protein containing PH domain; PTB domain and leucine zipper motif 2) (Biotin); PTB domain and leucine zipper motif 2; anti-APPL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 664

• Applicable Applications for anti-APPL2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: APPL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-152 amino acids from the N-terminal region of human APPL2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of APPL2 Antibody (N-term) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in NCI-H292 cell line lysate. APPL2 Antibody (N-term) (arrow) was detected using the purified Pab.)

Related Product Information for anti-APPL2 antibody

Required for the regulation of cell proliferation in response to extracellular signals mediated by an early endosomal compartment. Links Rab5 to nuclear signal transduction.

Product Categories/Family for anti-APPL2 antibody

Antibodies; Leucine Zipper Proteins

References

Chial, H.J., et al. PLoS ONE 5 (8), E12471 (2010): Rashid, S., et al. J. Biol. Chem. 284(27):18115-18128(2009). Chial, H.J., et al. Traffic 9(2):215-229(2008). Nechamen, C.A., et al. Mol. Cell. Endocrinol. 260-262, 93-99 (2007): Mao, X., et al. Nat. Cell Biol. 8(5):516-523(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 24586663
• NCBI GeneID: 55198
• NCBI Accession #: NP_060641.2
• NCBI GenBank Nucleotide #: NP_060641.2
• UniProt Accession #: Q8NEU8
• NCBI Official Full Name: DCC-interacting protein 13-beta isoform 1
• NCBI Official Synonym Full Names: adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 2
• NCBI Official Symbol: APPL2
• NCBI Official Synonym Symbols: DIP13B
• NCBI Protein Information: DCC-interacting protein 13-beta
• UniProt Protein Name: DCC-interacting protein 13-beta
• Protein Family: DCC-interacting protein
• UniProt Gene Name: APPL2
• UniProt Synonym Gene Names: DIP13B; Dip13-beta
• UniProt Entry Name: DP13B_HUMAN

NCBI Description

The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011]