Bovine Growth-associated protein 43 ELISA Kit | GAP-43 elisa kit

Bovine Growth-associated protein 43 ELISA Kit

Cat. Num. AAA744126

• Gene Names: GAP43; B-50; PP46
• Reactivity: Bovine
• Synonyms: Growth-associated protein 43; Bovine Growth-associated protein 43 ELISA Kit; GAP-43 elisa kit

• Reactivity: Bovine
• Sequence Length: 238

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This GAP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine GAP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GAP-43 elisa kit

Principle of the Assay: GAP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GAP antibody and an GAP-HRP conjugate. The assay sample and buffer are incubated together with GAP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAP concentration since GAP from samples and GAP-HRP conjugate compete for the anti-GAP antibody binding site. Since the number of sites is limited, as more sites are occupied by GAP from the sample, fewer sites are left to bind GAP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119600000
• NCBI GeneID: 2596
• UniProt Accession #: P17677; A8K0Y4; Q5U058; Q6LB24
• Molecular Weight: 24,803 Da
• NCBI Official Full Name: growth associated protein 43
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: GAP43
• NCBI Official Synonym Symbols: B-50; PP46
• NCBI Protein Information: neuromodulin; protein F1; neural phosphoprotein B-50; growth-associated protein 43; axonal membrane protein GAP-43; calmodulin-binding protein P-57; nerve growth-related peptide GAP43; neuron growth-associated protein 43
• UniProt Protein Name: Neuromodulin
• UniProt Gene Name: GAP43
• UniProt Entry Name: NEUM_HUMAN

NCBI Description

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: filopodium membrane; axon; postsynaptic density; cytoplasm; plasma membrane; cell junction

Molecular Function: calmodulin binding; protein binding

Biological Process: cell fate commitment; glial cell differentiation; tissue regeneration; regulation of growth; axon choice point recognition; protein kinase C activation; response to wounding; regulation of filopodium formation