Rabbit anti-Human GRO Beta/CXCL2 Polyclonal Antibody | anti-CXCL2 antibody

Anti-GRO Beta/CXCL2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8103273

• Gene Names: CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: GRO Beta/CXCL2; Polyclonal Antibody; Anti-GRO Beta/CXCL2 Antibody; Rabbit Polyclonal; CXCL2/MIP-2 Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-X-C motif) ligand 2; Anti-CINC-2a Antibody; Anti-GRO2 Antibody; Anti-GROb Antibody; Anti-MGSA-b Antibody; Anti-MIP-2a Antibody; Anti-MIP2 Antibody; Anti-MIP2A Antibody; Anti-SCYB2 Antibody; anti-CXCL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CXCL2/MIP-2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CXCL2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000
• Immunogen: Recombinant Human CXCL2/MIP-2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CXCL2/MIP-2 (rh CXCL2/MIP-2). CXCL2/MIP-2 specific IgG was purified by Human CXCL2/MIP-2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CXCL2 rabbit polyclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:11 kDaObserved band size:11 kDa)

Related Product Information for anti-CXCL2 antibody

Chemokine (C-X-C motif) ligand 2 (CXCL2), also called macrophage inflammatory protein 2 (MIP-2), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2), is a small cytokine belonging to the CXC chemokine family. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone, where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for CXCL2/MIP-2 or in the presence of a blocking Ab to CXCL2/MIP-2, peripheral tolerance is prevented, and Ag-specific T regulatory cells are not generated. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone, where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for MIP-2 (as in CXCR2-deficient mice) or in the presence of a blocking Ab to MIP-2, peripheral tolerance is prevented, and Ag-specific T regulatory cells are not generated. Understanding the regulation of lymphocyte traffic during tolerance induction may lead to novel therapies for autoimmunity, graft acceptance, and tumor rejection. Several studies have implicated the CXCL2 chemokine as a mediator in the development of sepsis. CXCL2/MIP-2 also plays a major role in mediating the neutrophilic inflammatory response of the rodent lung to particles such as quartz, crocidolite asbestos, as well as high doses of other relative innocuous dusts such as titanium dioxide.

Product Categories/Family for anti-CXCL2 antibody

Neuroinflammation; IL17 signaling pathway

References

Driscoll KE. (2000) TNFalpha and MIP-2: role in particle-induced inflammation and regulation by oxidative stress. Toxicol Lett. 112-113: 177-83.Walpen S, et al. (2001) Nitric oxide induces MIP-2 transcription in rat renal mesangial cells and in a rat model of glomerulonephritis. FASEB J. 15(3): 571-3.Fahey TJ, et al. (1990) Cytokine production in a model of wound healing: the appearance of MIP-1, MIP-2, cachectin/TNF and IL-1. Cytokine. 2(2): 92-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4504155
• NCBI GeneID: 2920
• NCBI Accession #: NP_002080.1
• NCBI GenBank Nucleotide #: NM_002089.3
• UniProt Accession #: P19875; Q6FGD6; Q9UPB8
• Molecular Weight: 11,389 Da
• NCBI Official Full Name: C-X-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 2
• NCBI Official Symbol: CXCL2
• NCBI Official Synonym Symbols: GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
• NCBI Protein Information: C-X-C motif chemokine 2; GRO2 oncogene; MGSA beta; MIP2-alpha; gro-beta; growth-regulated protein beta; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta
• UniProt Protein Name: C-X-C motif chemokine 2
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL2
• UniProt Synonym Gene Names: GRO2; GROB; MIP2A; SCYB2; Gro-beta; MIP2-alpha; HSF
• UniProt Entry Name: CXCL2_HUMAN

NCBI Description

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; response to molecule of bacterial origin; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; chemotaxis; inflammatory response; regulation of cell proliferation